Effect of curcumin-encapsulated Pluronic® F-127 over duo-species biofilm of Streptococcus mutans and Candida albicans

Autor: Diego Dantas Lopes dos Santos, João Felipe Besegato, Marlus Chorilli, João Augusto Oshiro Junior, Vanderlei Salvador Bagnato, Dongmei Deng, Priscila Borges Gobbo de Melo, Alessandra Nara de Souza Rastelli
Přispěvatelé: Universidade Estadual Paulista (UNESP), State University of Paraíba-UEPB, University of Amsterdam and Vrije Universiteit Amsterdam, Universidade de São Paulo (USP), Preventive Dentistry
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
dos Santos, D D L, Besegato, J F, de Melo, P B G, Junior, J A O, Chorilli, M, Deng, D, Bagnato, V S & de Souza Rastelli, A N 2022, ' Effect of curcumin-encapsulated Pluronic ® F-127 over duo-species biofilm of Streptococcus mutans and Candida albicans ', Lasers in Medical Science, vol. 37, no. 3, pp. 1775-1786 . https://doi.org/10.1007/s10103-021-03432-9
Lasers in Medical Science, 37(3), 1775-1786. Springer London
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 0268-8921
DOI: 10.1007/s10103-021-03432-9
Popis: Made available in DSpace on 2022-04-29T08:35:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-04-01 To assess the effect of curcumin-encapsulated Pluronic® F-127 (Cur-Plu) during antimicrobial photodynamic therapy (aPDT) over duo-species biofilm of Streptococcus mutans and Candida albicans. Thermal analysis, optical absorption, and fluorescence spectroscopy were evaluated. Minimum inhibitory concentration (MIC) and minimum bactericidal/fungal concentration were obtained. The biofilms were cultured for 48 h at 37 °C and treated according to the groups: P + M + L + (photosensitizer encapsulated with Pluronic® F-127 + light); P + D + L + (photosensitizer incorporated in 1% DMSO + light); P − M + L + (no Pluronic® F-127 + light); P − D + L + (1% DMSO + light); P − L + (Milli-Q water + light); P + M + L − (photosensitizer encapsulated with Pluronic® F-127 no light); P + D + L − (photosensitizer in 1% DMSO, no light); P − M + L − (Pluronic® F-127 no light); P − D + L − (1% DMSO, no light); P − L − (Milli-Q water, no light; negative control group); CHX (0.2% chlorhexidine, positive control group); and NYS (Nystatin). Dark incubation of 5 min was used. The groups that received aPDT were irradiated by blue LED (460 nm, 15 J/cm2). Cell viability of the biofilms was performed by colony-forming units (CFU/mL) and confocal microscopy. Two-way ANOVA followed by Tukey’s post hoc test was used at a significance level of 5%. P + D + L + and P + M + L + groups exhibited better log-reduction for both Candida albicans and Streptococcus mutans biofilms than P − M + L + , P − L + , and P − D + L + experimental groups. Furthermore, P + M + L + and P + D + L + showed greater reduction for Candida albicans than for Streptococcus mutans. aPDT mediated by Cur-Plu can be a potential strategy for biofilm control against duo-species biofilm of Streptococcus mutans and Candida albicans. Department of Dental Materials and Prosthodontics School of Dentistry Araraquara São Paulo State University – UNESP, São Paulo Department of Restorative Dentistry School of Dentistry Araraquara São Paulo State University – UNESP, 1680 Humaitá St., MailBox: 331, São Paulo Department of Drugs and Medicines School of Pharmaceutical Sciences Araraquara São Paulo State University – UNESP, São Paulo Graduate Program in Pharceutical Sciences State University of Paraíba-UEPB Department of Preventive Dentistry Academic Centre for Dentistry Amsterdam – ACTA University of Amsterdam and Vrije Universiteit Amsterdam Department of Physics and Materials Science Physics Institute of São Carlos - IFSC University of São Paulo – USP, São Paulo Department of Dental Materials and Prosthodontics School of Dentistry Araraquara São Paulo State University – UNESP, São Paulo Department of Restorative Dentistry School of Dentistry Araraquara São Paulo State University – UNESP, 1680 Humaitá St., MailBox: 331, São Paulo Department of Drugs and Medicines School of Pharmaceutical Sciences Araraquara São Paulo State University – UNESP, São Paulo
Databáze: OpenAIRE
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