Heat treatment of serum samples from stray dogs naturally exposed to Dirofilaria immitis and Dirofilaria repens in Romania
| Autor: | Laura Rinaldi, L. Del Prete, Giuseppe Cringoli, Laura D. Kramer, Lavinia Ciuca, Liviu Miron, M.P. Maurelli, Carlo Mangia, Marco Genchi |
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| Přispěvatelé: | Società Italiana di Parassitologia (SOIPA), Ciucă, L, Genchi, M, Kramer, L, Mangia, C, Miron, L, DEL PRETE, Luisa, Maurelli, MARIA PAOLA, Cringoli, Giuseppe, Rinaldi, Laura, Ciucă, L., Genchi, M., Mangia, C., Miron, L. D. |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Veterinary medicine Hot Temperature Dirofilaria immitis Occult infection 030231 tropical medicine Population Enzyme-Linked Immunosorbent Assay Antigen test Polymerase Chain Reaction Repens 03 medical and health sciences Dogs 0302 clinical medicine Antigen Pre-treatment heat Animals Helminths Dog Diseases education False Negative Reactions Dirofilaria immiti Diagnostic Techniques and Procedures education.field_of_study General Veterinary biology Romania Dirofilaria repen General Medicine 030108 mycology & parasitology biology.organism_classification Serum samples Dirofilaria repens Parasitology Antigens Helminth Veterinary (all) Dirofilariasis |
| Zdroj: | Veterinary Parasitology. 225:81-85 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/j.vetpar.2016.05.032 |
| Popis: | Pre-heating of serum samples has been shown to reverse false negative antigen tests for Dirofilaria immitis infection in dogs. Here the authors report the results of serum sampling in a population of dogs naturally exposed to D. immitis and Dirofilaria repens infection by testing in ELISA before and after heat treatment. Of 194 dogs sampled from four cities in Romania, D. immitis circulating antigens were found in 16 (8.2%) non heated samples and in 52 (26.8%) heated samples. Of the 108 dogs examined by Knott test, 24 dogs (22.2%) were positive for circulating mf. Subsequent PCR identification showed six dogs had D. immitis mf only, 12 dogs, had only D. repens mf, and 5 were positive for both. Fifty% of dogs with circulating D. immitis mf had positive antigen tests before and after heating, while the other 50% reverted to positive only after heat treatment. Sixty% of dogs with mixed D. immitis/D. repens infection were antigen positive before and after heating, while the other 40% converted to positive after heating. Antigen testing for D. immitis in the 12 dogs with only D. repens mf gave conflicting results. Only two dogs (16%) were antigen negative both before and after heat treatment. Six dogs (50%) became antigen positive after heating and four dogs (30%) were antigen positive both before and after heat treatment. Results would suggest that: false negative result for antigen testing can be reverted by heating of the serum sample; dogs infected with D. repens may have also an occult infection with D. immitis; heat treatment of serum from D. repens-infected dogs can reveal an occult infection with D. immitis. |
| Databáze: | OpenAIRE |
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