Upregulation of tumor necrosis factor-alpha production by retrovirally transduced human tumor-infiltrating lymphocytes using trans-retinoic acid
| Autor: | Patrick Hwu, R Cowherd, J. Treisman, G. E. Shafer, John R. Yannelli, Steven A. Rosenberg, Dvorit Samid |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Genetic enhancement
medicine.medical_treatment Immunology Genetic Vectors Retinoic acid Gene Expression Tretinoin chemistry.chemical_compound Lymphocytes Tumor-Infiltrating Downregulation and upregulation Transduction Genetic Gene expression Murine leukemia virus medicine Humans RNA Messenger Repetitive Sequences Nucleic Acid biology Tumor-infiltrating lymphocytes Tumor Necrosis Factor-alpha biology.organism_classification Up-Regulation Cytokine chemistry Cancer research Tumor necrosis factor alpha Moloney murine leukemia virus |
| Zdroj: | Cellular immunology. 156(2) |
| ISSN: | 0008-8749 |
| Popis: | The ability of retinoic acid (RA) to upregulate gene expression in human tumor-infiltrating lymphocytes (TIL) transduced with a Moloney murine leukemia virus containing the cDNA encoding tumor necrosis factor (TNF) has been studied. TNF production was increased approximately twofold after treatment with RA. This increase was dose dependent and corresponded to a rise in the level of LTR-driven mRNA measured by Northern analysis. RA did not appreciably increase transcription by the SV40 promoter or increase endogenous TNF production. The effect lasted for 3-6 days following withdrawal of RA. These studies indicate that RA can upregulate LTR-driven gene expression in TIL cells bearing retroviral vectors and may thus be of use in studies of the gene therapy of cancer. |
| Databáze: | OpenAIRE |
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