Cross-neutralization between three mumps viruses & mapping of haemagglutinin-neuraminidase (HN) epitopes
| Autor: | Sunil R. Vaidya, Santoshkumar M. Jadhav, Garima M Dvivedi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology medicine.diagnostic_test business.industry 030106 microbiology lcsh:R lcsh:Medicine General Medicine Mumps virus medicine.disease_cause Virology General Biochemistry Genetics and Molecular Biology Epitope Microbiology Cross-neutralization - India - mumps wild types - mumps Leningrad-Zagreb vaccine strain 03 medical and health sciences Mumps vaccine Immunity Immunoassay Genotype biology.protein medicine HN Protein Neutralizing antibody business |
| Zdroj: | Indian Journal of Medical Research, Vol 143, Iss 1, Pp 37-42 (2016) |
| ISSN: | 0971-5916 |
| Popis: | Background & objectives: The reports from the countries where mumps vaccine is given as routine immunization suggest differences in mumps virus neutralizing antibody titres when tested with vaccine and wild type viruses. Such reports are unavailable from countries like India where mumps vaccine is not included in routine immunization. We, therefore, undertook this study to understand the cross-neutralization activity of Indian mumps viruses. Methods: By using commercial mumps IgG enzyme immunoassay (EIA) and a rapid focus reduction neutralization test (FRNT), a panel of serum samples was tested. The panel consisted of 14 acute and 14 convalescent serum samples collected during a mumps outbreak and 18 archived serum samples. Two wild types (genotypes C and G) and Leningrad-Zagreb vaccine strain (genotype N) were used for the challenge experiments and FRNT titres were determined and further compared. The HN protein sequence of three mumps viruses was analyzed for the presence of key epitopes. Results: All serum samples effectively neutralized mumps virus wild types and a vaccine strain. However, significantly lower FRNT titres were noted to wild types than to vaccine strain (P |
| Databáze: | OpenAIRE |
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