Expression of morphogenic genes in mature ovarian and testicular tissues: Potential stem-cell niche markers and patterning factors
| Autor: | William S. Davidson, Ben F. Koop, Matthew L. Rise, Sean P. McCarthy, James C. Hutson, Kristian R. von Schalburg |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Cell type Frizzled animal structures Xenopus Molecular Sequence Data Ovary Biology Transcriptome Mice Testis Morphogenesis Genetics medicine Animals Humans Cell Lineage Amino Acid Sequence Body Patterning Oligonucleotide Array Sequence Analysis Stem Cells Wnt signaling pathway Gene Expression Regulation Developmental Cell Biology Embryonic stem cell Rats Cell biology Mice Inbred C57BL medicine.anatomical_structure Oncorhynchus mykiss embryonic structures Female Seasons Stem cell Sequence Alignment Developmental Biology Morphogen |
| Zdroj: | Molecular Reproduction and Development. 73:142-152 |
| ISSN: | 1098-2795 1040-452X |
| DOI: | 10.1002/mrd.20359 |
| Popis: | Morphogens are developmental regulators that modulate different tissue patterning, proliferation, differentiation, or remodeling processes in embryonic and adult tissues. Morphogens may also evoke specific regulatory programs in stem cells. Some of the morphogens involved in these processes have been characterized, while others remain unidentified. A microarray containing 3,557 salmonid cDNAs was used to compare the transcriptomes of rainbow trout precocious ovary at three different stages during second year (June, August, and October) with a reference (June normal ovary) transcriptome. During this study, we detected morphogen transcript hybridizations to salmonid elements and the study was enlarged to investigate these activities in various developmental stages of both ovary and testis. Genes from diverse development regulator families such as Anterior gradient-2, BMP, Epimorphin, Flightless, Frizzled, Notch, Tiarin, Twisted gastrulation, and Wnt were demonstrated to be expressed in the adult trout gonads. In mice or rats, expression of mammalian bmp-4, epimorphin, flightless, twisted gastrulation, and GW112 transcripts were localized to cell types isolated from the developed ovary and testis. Comparisons of salmonid and mammalian morphogens at the amino acid residue level show high similarities, suggesting functional conservation. This report provides evidence for local regulation by various morphogens and their potential to control distinct programs of gene expression in the gametes and their accessory cells during gametogenesis. Mol. Reprod. Dev. © 2005 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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