Development of an up-converting phosphor technology-based 10-channel lateral flow assay for profiling antibodies against Yersinia pestis

Autor: Huijie Huang, Lei Zhou, Yanfeng Yan, Lihua Huang, Zongmin Du, Jianfeng Qu, Ziwen Zhu, Zhaobiao Guo, Wenyan Hong, Zheng Yan, Ruifu Yang, Youbao Zhang, Haoran Wang, Chengke Xie
Rok vydání: 2010
Předmět:
Zdroj: Journal of Microbiological Methods. 83:133-140
ISSN: 0167-7012
DOI: 10.1016/j.mimet.2010.08.005
Popis: article i nfo Article history: In this study, a 10-channel up-converting phosphor technology-based lateral flow (TC-UPT-LF) assay was developed to profile antibodies against Yersinia pestis. Ten expressed Y. pestis proteins were covalently conjugated with an up-converting phosphor particle to develop double-antigen sandwich immunochroma- tographic strips to detect corresponding antibodies. After optimization one by one, each strip was integrated into a TC-UPT-LF disc for simultaneously detection of different antibodies. A scanning biosensor was also developed to acquire the results. The performance of the TC-UPT-LF assay was evaluated by using standard samples and plague monkey serum samples. Fifty-one patient serum samples were detected by the TC-UPT- LF assay. The TC-UPT-LF disc could be stable for 10 days at 37 °C with an average CV of 10.3%. Its sensitivity and qualitative results are comparable to those of ELISA. Its linearity fitting coefficient of determination (R 2 ) for different antibody detection is between 0.93 and 0.99. Besides F1 antibody, the LcrV and YopD antibodies also showed higher positive ratio than the other seven antibodies, as 100% (13/13) and 92% (12/13) in monkey sera and 86.3% (44/51) and 66.7% (34/51) in patient sera, respectively. It is suggested that the TC- UPT-LF assay has been successfully developed for multi-detection and LcrV and YopD can be the potential diagnostic markers of the plague.
Databáze: OpenAIRE
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