Global transcriptome study of Dip2B-deficient mouse embryonic lung fibroblast reveals its important roles in cell proliferation and development
| Autor: | Yang Chen, Zin Mar Oo, Salah Adlat, Mahmoud Al-Azab, Yaowu Zheng, Xuechao Feng, Farooq Hayel, May Zun Zaw Myint, Luqing Zhang, Fatoumata Binta Bah, Rajiv Kumar Sah, MI Nasser, Noor Bahadar |
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| Rok vydání: | 2020 |
| Předmět: |
Protein digestion
lcsh:Biotechnology Cellular differentiation Biophysics Biochemistry Transcriptome 03 medical and health sciences 0302 clinical medicine Structural Biology lcsh:TP248.13-248.65 microRNA Genetics DEG Progenitor cell KEGG Transcription factor ComputingMethodologies_COMPUTERGRAPHICS 030304 developmental biology 0303 health sciences Chemistry Computer Science Applications Cell biology Gene expression profiling MELFs 030220 oncology & carcinogenesis Gene ontology Dip2B knockout Research Article Biotechnology |
| Zdroj: | Computational and Structural Biotechnology Journal, Vol 18, Iss, Pp 2381-2390 (2020) Computational and Structural Biotechnology Journal |
| ISSN: | 2001-0370 |
| Popis: | Graphical abstract Disco-interacting protein 2 homolog B (Dip2B) is a member of Dip2 family encoded by Dip2b gene. Dip2B has been reported to regulate murine epithelial KIT+ progenitor cell expansion and differentiation epigenetically via exosomal miRNA targeting during salivary gland organogenesis. However, its molecular functions, cellular activities and biological process remain unstudied. Here, we investigated the transcriptome of Dip2B-deficient mouse embryonic lung fibroblasts (MELFs) isolated from E14.5 embryos by RNA-Seq. Expression profiling identified 1369 and 1104 differentially expressed genes (DEGs) from Dip2b−/− and Dip2b+/− MELFs in comparisons to wild-type (Dip2b+/+). Functional clustering of DEGs revealed that many gene ontology terms belong to membrane activities such as ‘integral component of plasma membrane’, and ‘ion channel activity’, suggesting possible roles of Dip2B in membrane integrity and membrane function. KEGG pathway analysis revealed that multiple metabolic pathways are affected in Dip2b−/− and Dip2b+/− when compared to Dip2b+/+ MELFs. These include ‘protein digestion and absorption’, ‘pancreatic secretion’ and ‘steroid hormone synthesis pathway’. These results suggest that Dip2B may play important roles in metabolism. Molecular function analysis shows transcription factors including Hox-genes, bHLH-genes, and Forkhead-genes are significantly down-regulated in Dip2b−/− MELFs. These genes are critical in embryo development and cell differentiation. In addition, Dip2B-deficient MELFs demonstrated a reduction in cell proliferation and migration, and an increase in apoptosis. All results indicate that Dip2B plays multiple roles in cell proliferation, migration and apoptosis during embryogenesis and may participate in control of metabolism. This study provides valuable information for further understanding of the function and regulatory mechanisms of Dip2B. |
| Databáze: | OpenAIRE |
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