Selective destruction of amino acid residues in irradiated solutions of superoxide dismutase
| Autor: | G. Rotilio, Donatella Barra, E. Martin Fielden, Peter B. Roberts, Lilia Calabrese, Francesco Bossa |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Free Radicals
Radical Lysine Biophysics chemistry.chemical_element Zinc Biochemistry Medicinal chemistry Superoxide dismutase Animals Amino Acids Binding site Molecular Biology chemistry.chemical_classification Binding Sites Bromine biology Superoxide Dismutase Chemistry Cell Biology Hydrogen-Ion Concentration Copper Amino acid Radiation Effects biology.protein Cattle Apoproteins Hydrogen Protein Binding |
| Zdroj: | Biochemical and Biophysical Research Communications. 64:1303-1309 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(75)90834-7 |
| Popis: | Amino acid analyses of irradiated bovine superoxide dismutase solutions showed that only a few types of residues are destroyed by H and Br 2 − radicals and confirmed the indications obtained from work on free amino acids. Furthermore destruction of lysine - unexpected on the basis of data obtained in free solutions of amino acids exposed to Br 2 − - was observed in the copper-containing protein. Different extent of amino acid losses were observed depending on pH and presence or absence of copper. Correlation of these losses with residual enzymic activity permitted identification of some vital residues. |
| Databáze: | OpenAIRE |
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