Determining the optimal storage time and temperature for performing platelet function assays and global hemostasis assays
| Autor: | M.L.J. Jeurissen, Paul W.M. Verhezen, E.J.S. Denessen, Rick J. H. Wetzels, D L Van Den Kerkhof, Yvonne M. C. Henskens |
|---|---|
| Přispěvatelé: | Faculteit FHML Centraal, RS: Carim - B01 Blood proteins & engineering, MUMC+: DA CDL Analytisch cluster 1K (9), MUMC+: DA CDL Algemeen (9), RS: NUTRIM - R2 - Liver and digestive health, RS: Carim - B04 Clinical thrombosis and Haemostasis |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male 0301 basic medicine Light transmission Platelet Function Tests Screening test Information Storage and Retrieval MULTIPLATE 030204 cardiovascular system & hematology VARIABLES Young Adult 03 medical and health sciences 0302 clinical medicine Healthy volunteers WHOLE-BLOOD MANAGEMENT Humans Platelet Global haemostasis assays Whole blood Hemostasis Chemistry Temperature optimal storage temperature Hematology General Medicine Thromboelastometry 030104 developmental biology pre-analytical variables platelet function assays maximal storage times Biological Assay Female Bleeding tendencies Biomedical engineering |
| Zdroj: | Platelets, 33(3), 416-424. Routledge/Taylor & Francis Group |
| ISSN: | 1369-1635 0953-7104 |
| Popis: | Platelet function assays and global haemostasis assays are essential in diagnosing bleeding tendencies, with light transmission aggregometry (LTA) as golden standard. The Multiple Electrode Aggregation (Multiplate), platelet function assay (PFA) and rotational thromboelastometry (ROTEM) are mostly used as whole-blood screening tests. Currently, patients have to travel to specialized laboratories to undergo these tests, since specific expertise is required. Pre-analytical variables, like storage time and temperature during transport, are still considered to be the most vulnerable part of the process and may lead to discrepancies in the test results. We aim to give a first impression on the stability of blood samples from healthy volunteers during storage and investigate the effect of storage time (1, 3, 6 and 24 hours) and temperature (4 degrees C, room temperature and 37 degrees C) on the Multiplate, PFA, ROTEM and LTA test results. Our data indicated that, for the PFA, whole blood can be stored for 3 hours at room temperature. Whole blood used for the Multiplate and ROTEM can be stored for 6 hours of storage. For LTA, PRP and whole blood were stable up to 3 hours at 4 degrees C or room temperature and 6 hours at room temperature, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|