Isolation, purification and antioxidant activities of polysaccharides from Grifola frondosa
| Autor: | Guo-qiang Zhao, Sheng-tao Liu, Guitang Chen, Xue-mei Ma, Yan-li Liao |
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| Rok vydání: | 2012 |
| Předmět: |
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Antioxidant Polymers and Plastics DPPH medicine.medical_treatment Polysaccharide Antioxidants Rats Sprague-Dawley chemistry.chemical_compound Lipid oxidation Picrates Superoxides Materials Chemistry medicine Animals Ferrous Compounds Fruiting Bodies Fungal Ferricyanides Grifola frondosa Chelating Agents chemistry.chemical_classification Chromatography biology Hydroxyl Radical fungi Organic Chemistry Biphenyl Compounds Fungal Polysaccharides Grifola biology.organism_classification chemistry Biochemistry Liver Sephadex Hydroxyl radical Lipid Peroxidation Oxidation-Reduction |
| Zdroj: | Carbohydrate polymers. 89(1) |
| ISSN: | 1879-1344 |
| Popis: | The crude polysaccharides (GFP) were isolated from the fruiting bodies of Grifola frondosa and purified by DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography in that order. Three main fractions, GFP-1, GFP-2 and GFP-3, were obtained through the isolation and purification steps. Then the antioxidant activities of these three fractions were investigated in vitro. The results showed that GFP-1, GFP-2 and GFP-3 possessed significant inhibitory effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical; their reducing power, ferrous ions chelating effect and the inhibition ability of the rat liver lipid oxidation where also strong. These results suggest that G. frondosa polysaccharides could be a suitable natural antioxidant and may be the functional foods for humans. |
| Databáze: | OpenAIRE |
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