The INO80 Protein Controls Homologous Recombination in Arabidopsis thaliana
| Autor: | Jean Molinier, Barbara Hohn, Olivier Fritsch, Chris Bowler, Giovanna Benvenuto |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial 0106 biological sciences Saccharomyces cerevisiae Proteins Transcription Genetic DNA repair Mutant Arabidopsis 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Transcriptional regulation Transcription factor Gene Molecular Biology 030304 developmental biology Recombination Genetic Genetics 0303 health sciences biology Arabidopsis Proteins Cell Biology Plants Genetically Modified biology.organism_classification Chromatin Mutation Homologous recombination Mutagens Transcription Factors 010606 plant biology & botany |
| Zdroj: | Molecular Cell. 16(3):479-485 |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/j.molcel.2004.09.034 |
| Popis: | Homologous recombination (HR) serves a dual role in providing genetic flexibility and in maintaining genome integrity. Little is known about the regulation of HR and other repair pathways in the context of chromatin. We report on a mutant affected in the expression of the Arabidopsis INO80 ortholog of the SWI/SNF ATPase family, which shows a reduction of the HR frequency to 15% of that in wild-type plants. In contrast, sensitivity to genotoxic agents and efficiency of T-DNA integration remain unaffected, suggesting that INO80 is a positive regulator of HR, while not affecting other repair pathways. So far, INO80 function has only been reported in a lower eukaryote. Profiling studies on three ino80 allelic mutants show that INO80 regulates nearly 100 Arabidopsis genes. However, the transcriptional regulation of repair-related genes is unaffected in the mutant. This suggests a dual role for INO80 in transcription and DNA repair by HR. |
| Databáze: | OpenAIRE |
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