Leydig cell loss and spermatogenetic arrest in platelet-derived growth factor (PDGF)-A-deficient mice
| Autor: | Chiayeng Wang, Stefania Mariani, Linda Karlsson, Sabrina Basciani, Giovanni Spera, Cecilia Bondjers, Lucio Gnessi, Mario Arizzi, Christer Betsholtz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Male
Platelet-derived growth factor medicine.medical_treatment Apoptosis gene targeting chemistry.chemical_compound Mice 0302 clinical medicine Leydig cell Receptors Platelet-Derived Growth Factor In Situ Hybridization Mice Knockout Platelet-Derived Growth Factor 0303 health sciences biology Brief Report Leydig Cells PDGF-A 3. Good health medicine.anatomical_structure Seminiferous Epithelium 030220 oncology & carcinogenesis platelet derived growth factor testis Platelet-derived growth factor receptor medicine.medical_specialty endocrine system 03 medical and health sciences Internal medicine medicine In Situ Nick-End Labeling Animals RNA Messenger 030304 developmental biology urogenital system Growth factor Mesenchymal stem cell Leydig cell differentiation Cell Biology Epithelium spermatogenesis Mice Inbred C57BL Endocrinology chemistry Animals Newborn Bromodeoxyuridine biology.protein Spermatogenesis |
| Zdroj: | The Journal of Cell Biology |
| Popis: | Platelet-derived growth factor (PDGF)- A–deficient male mice were found to develop progressive reduction of testicular size, Leydig cells loss, and spermatogenic arrest. In normal mice, the PDGF-A and PDGF-Rα expression pattern showed positive cells in the seminiferous epithelium and in interstitial mesenchymal cells, respectively. The testicular defects seen in PDGF-A−/− mice, combined with the normal developmental expression of PDGF-A and PDGF-Rα, indicate that through an epithelial-mesenchymal signaling, the PDGF-A gene is essential for the development of the Leydig cell lineage. These findings suggest that PDGF-A may play a role in the cascade of genes involved in male gonad differentiation. The Leydig cell loss and the spermatogenic impairment in the mutant mice are reminiscent of cases of testicular failure in man. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|