Generation of porcine Pasteurella multocida ghost vaccine and examination of its immunogenicity against virulent challenge in mice
| Autor: | Hong-Li Geng, Hongbo Ni, Xiang-Zhu Meng, Xuhua Ran, Chunlong Chang, Xiaobo Wen, Xiaohong Chen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Serotype Pasteurella multocida Swine medicine.medical_treatment Pasteurella Infections 030106 microbiology Microbiology Lethal Dose 50 Interferon-gamma Mice Viral Proteins 03 medical and health sciences Immunogenicity Vaccine Adjuvants Immunologic Antigen medicine Animals biology business.industry Immunogenicity biology.organism_classification Antibodies Bacterial Virology Bacterial Load Vaccination Disease Models Animal Titer 030104 developmental biology Infectious Diseases Vaccines Inactivated Bacterial Vaccines Inactivated vaccine Immunization Interleukin-4 business Adjuvant Spleen |
| Zdroj: | Microbial Pathogenesis. 132:208-214 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2019.04.016 |
| Popis: | Pasteurella multocida (PM) causes a varity of clinical manifestation in domestic animals, even acute death. Vaccination is among effective strategy to prevent and control PM-related diseases. Bacterial ghosts (BGs) are empty bacterial envelopes, which sustain subtle antigenic comformation in bacterial outer-membrane and exhibit higher efficacy compared to inactivated vaccines. Here, a BG vaccine generated from the porcine PM reference strain CVCC446 (serotype B:2) was prepared upon lysis by E protein of bacteriophage PhiX174, and the safety and immunogenicity were evaluated its in a mouse model. Lysis rate was in 99.99% and the BG vaccine was completely inactivated by addition of freeze-dry procedure. Mice were immunized subcutaneously twice in 2-week intervals with BGs, or BGs plus adjuvant, or formalin-inactivated PM or an adjuvant control. Mice inoculated twice with BGs vaccines generated higher titer of antibodies, interleukin 4 and gamma interferon than those in the inactivated vaccine group or adjuvant placebo group (P 0.05). CD4 |
| Databáze: | OpenAIRE |
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