Microbiological surveillance post-reprocessing of flexible endoscopes used in digestive endoscopy: a national study

Autor: B. Casini, A.M. Spagnolo, M. Sartini, B. Tuvo, M. Scarpaci, M. Barchitta, A. Pan, A. Agodi, M.L. Cristina, L. Baroncelli, P. Castiglia, M. De Giusti, M.M. Distefano, A.M. Longhitano, P. Laganà, B. Mentore, F. Canale, F. Mantero, M. Opezzi, E. Marciano, L. Zurlo, A. Segata, I. Torre, D. Vay, E. Vecchi, S. Vincenti
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Popis: Microbiological surveillance of endoscopes is a safety measure for verifying the quality of reprocessing procedures and identifying contaminated devices, but duodenoscope-related outbreaks are still reported.To assess the effectiveness of duodenoscope reprocessing procedures in Italy.Between December 2019 and April 2020, data obtained from microbiological surveillance post-reprocessing in 15 Italian endoscopy units were collected. Sampling was carried out after reprocessing or during storage in a cabinet. In keeping with international guidelines and the Italian position paper, the micro-organisms were classified as high-concern organisms (HCOs) and low-concern organisms (LCOs).In total, 144 samples were collected from 51 duodenoscopes. Of these, 36.81% were contaminated: 22.92% were contaminated with HCOs and 13.89% were contaminated with LCOs [2.08% with an LCO load of 11-100 colony-forming units (CFU)/device and 0.69% with an LCO load of100 CFU/device]. The contamination rate was 27.5% in samples collected after reprocessing, 40% in samples collected during storage in a cabinet that was compliant with EN 16442:2015 (C-I), and 100% in samples collected during storage in a cabinet that was not compliant with EN 16442:2015 (NC-I). The respective HCO rates were 15.00%, 27.27% and 66.67%. Correlation between LCO contamination and storage time was demonstrated (Spearman's rho=0.3701; P=0.0026). The Olympus duodenoscope TJFQ180V demonstrated the lowest rate of contamination (29.82%), although the contamination rate was 100% for duodenoscopes stored in an NC-I cabinet.Microbiological surveillance, along with strict adherence to reprocessing protocols, may help to detect endoscope contamination at an early stage, and reduce the risk of duodenoscope-associated infections.
Databáze: OpenAIRE