Methods of Biotyping of Streptococcus mutans Species with the Routine Test as a Prognostic Value in Early Childhood Caries
| Autor: | Krzyściak, Wirginia, Kościelniak, Dorota, Papież, Monika, Jurczak, Anna, Vyhouskaya, Palina |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Article Subject Teicoplanin business.industry medicine.drug_class 030106 microbiology Antibiotics Cefazolin Dentistry Erythromycin lcsh:Other systems of medicine Biology Amoxicillin Dental plaque medicine.disease biology.organism_classification lcsh:RZ201-999 Streptococcus mutans Microbiology 03 medical and health sciences Complementary and alternative medicine medicine business Early childhood caries medicine.drug |
| Zdroj: | Evidence-Based Complementary and Alternative Medicine, Vol 2017 (2017) |
| Popis: | Purpose. In order to investigate the suitability of Streptococcus mutans species biotyping by measuring the activity of selected enzymes from a commercial test, criteria were established for biotyping clinical strains from children with dental caries. In addition, the relationships between the selected biotypes, sensitivity to commonly used antibiotics, and early childhood caries were determined. Methods. A total of 142 S. mutans isolates from dental plaque of children with caries were divided into different biotypes. Patients were divided into two groups: noncavitated (1-2 in ICDAS) and cavitated (5-6 in ICDAS) lesions. Biotyping criteria were determined based on both the arbitrary method and the clusterization method. The susceptibility of the strains to amoxicillin, cefazolin, erythromycin, and teicoplanin was studied by diluting a solid medium. Results. Biotype I was the most common. Mean MIC values showed that the strains belonging to biotypes II and IV were the most sensitive to amoxicillin. For predetermined biotypes, observed differences were dependent on the severity of dental caries. Conclusions. The proposed method of S. mutans strains biotyping is relatively quick and simple to use, provided the application of suitable biotyping criteria, and may contribute to the effective prevention of dental caries induced by S. mutans. |
| Databáze: | OpenAIRE |
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