Syndecan-1 knockdown in endometrial epithelial cells alters their apoptotic protein profile and enhances the inducibility of apoptosis
| Autor: | Sarah Jean Boeddeker, D.M. Baston-Buest, J.S. Kruessel, Olga Altergot-Ahmad, Alexandra P. Hess |
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| Rok vydání: | 2014 |
| Předmět: |
Embryology
HSP27 Heat-Shock Proteins Protein Array Analysis Apoptosis Syndecan 1 Cell Line Endometrium Survivin Genetics medicine Humans HSP70 Heat-Shock Proteins Embryo Implantation Molecular Biology Clusterin biology Caspase 3 Obstetrics and Gynecology Trophoblast Epithelial Cells Cell Biology Cell biology medicine.anatomical_structure Reproductive Medicine Gene Expression Regulation Cell culture embryonic structures Cancer cell Heme Oxygenase (Decyclizing) biology.protein Cytokines Tumor necrosis factor alpha Female Syndecan-1 Developmental Biology Signal Transduction |
| Zdroj: | Molecular human reproduction. 20(6) |
| ISSN: | 1460-2407 |
| Popis: | Endometrial epithelial cells are known to undergo apoptosis during trophoblast invasion. We postulate that the cell surface molecule Syndecan-1 which is expressed on endometrial cells and syncytiotrophoblast is important for implantation in general and especially for induction of maternal cell apoptosis during trophoblast invasion because Syndecan-1's influence on apoptotic susceptibility of cancer cells is already described in the literature. Using the human endometrial epithelial cell line RL95-2, a new stable cell line with Syndecan-1 knockdown was generated. Via antibody array analysis, a significant decrease in the expression of anti-apoptotic proteins like inhibitors of apoptosis, Clusterin, heme oxygenase (HO-2), heat shock protein (HSP)27 and -70 and Survivin due to the Syndecan-1 knockdown was discovered. Correspondingly, active Caspase-3 as an indicator for apoptosis was increased more severely in these cells compared with unmodified RL95-2 after treatment with implantation-related stimuli, which are the cytokines interleukin-1β, interferon-γ, tumor necrosis factor-α and transforming growth factor-β1 and an anti-Fas antibody. Furthermore, a treatment with a combination of all factors caused a higher Caspase-3 induction compared with each single treatment. These results demonstrate that Syndecan-1 is involved in the control of apoptosis in RL95-2 cells and therefore may affect the fine tuning of apoptosis in endometrial epithelium regulating the embryo's invasion depth as a crucial step for regular implantation followed by successful pregnancy. |
| Databáze: | OpenAIRE |
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