Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria
| Autor: | Myrna Guest, Elizabeth Fox-Carter, K. S. Kirby |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
DNA
Bacterial Hot Temperature Lysis General Mathematics Bacillus subtilis Biology Nucleic Acid Denaturation medicine.disease_cause chemistry.chemical_compound Escherichia coli medicine Chemical Precipitation Applied Mathematics RNA Articles Ribosomal RNA biology.organism_classification RNA Bacterial chemistry Biochemistry Nucleic acid Ribosomes Ultracentrifugation DNA Bacteria |
| Zdroj: | Biochemical Journal. 104:258-262 |
| ISSN: | 0006-2936 |
| DOI: | 10.1042/bj1040258 |
| Popis: | 1. Nucleic acids were released from Escherichia coli by lysing with tri-iso-propylnaphthalene sulphonate and 4-aminosalicylate and then extracting with a phenol-cresol mixture. 2. Nucleic acids were similarly released from Bacillus subtilis after initial treatment with lysozyme. 3. DNA was sedimented after careful precipitation with m-cresol or 2-butoxyethanol (0.1-0.12vol.) in the presence of 20% sodium benzoate. 4. Contaminating ribosomal RNA was removed by precipitation in the presence of 4m-sodium chloride or by extracting DNA with an acetate-butyrate mixture, in which RNA is insoluble. 5. The DNA from B. subtilis has a transforming ability of 0.3-0.6% for the tryptophan marker. 6. Ribosomal RNA was then precipitated with rapidly labelled RNA by the addition of an equal volume of 2-butoxyethanol. 7. There was good separation of the nucleic acids from protein and polysaccharides. |
| Databáze: | OpenAIRE |
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