Microfluidic water-assisted trap focusing method for ultra-large volume injection in reversed-phase nano-liquid chromatography coupled to electron ionization tandem-mass spectrometry
| Autor: | Veronica Termopoli, Gian Luca Morini, Pierangela Palma, Mohammad Saeed, Achille Cappiello, Giorgio Famiglini, Pamela Vocale, Priscilla Rocío-Bautista, Simon J. Perry |
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| Přispěvatelé: | Termopoli, V, Famiglini, G, Vocale, P, Morini, G, Palma, P, Rocío-Bautista, P, Saeed, M, Perry, S, Cappiello, A, Termopoli V., Famiglini G., Vocale P., Morini G.L., Palma P., Rocio-Bautista P., Saeed M., Perry S., Cappiello A. |
| Rok vydání: | 2020 |
| Předmět: |
Acetonitriles
Microfluidics Ultra-large-volume injection Micromixer Electrons 010402 general chemistry Tandem mass spectrometry 01 natural sciences Biochemistry Analytical Chemistry Matrix (chemical analysis) Diffusion CHIM/01 - CHIMICA ANALITICA Limit of Detection Tandem Mass Spectrometry Nano-LC Microfluidic devices LC-MS LEI-MS Pesticides Liquid electron ionization (LEI)-tandem mass spectrometry Electron ionization Detection limit Chromatography Reverse-Phase Chromatography Chemistry 010401 analytical chemistry Organic Chemistry Selected reaction monitoring Reproducibility of Results Water General Medicine Water-assisted trap focusing 0104 chemical sciences Dilution Triple quadrupole mass spectrometer Pesticide Microfluidic Solvents Nanoparticles |
| Zdroj: | Journal of chromatography. A. 1627 |
| ISSN: | 1873-3778 |
| Popis: | Herein we present an efficient, column-switching method that relies on a custom-made T-union passive diffusion micromixer to assist water dilution and promote trap solute focusing of a high sample volume dissolved in pure organic solvent using a 0.075 mm i.d. nano-LC column. This method allows injecting 20 μL (or higher) of sample volume, speeding up the analysis time, with a 400-fold increase of the limits of quantitation for selected compounds. Five pesticides in different media were used as model compounds, and the analyses were carried out with a triple quadrupole mass spectrometer equipped with a Liquid Electron Ionization (LEI) LC-MS interface working in multiple reaction monitoring (MRM) mode. The system microfluidics were investigated using COMSOL modeling software. Robustness of the entire system was evaluated using a post-extraction addition soil extracts with limits of detection values spanning from 0.10 to 0.45 µg/L. Reproducible results in terms of peak area, peak shape, and retention times were achieved in soil matrix. Repeatability test on peak area variations were lower than 10%. |
| Databáze: | OpenAIRE |
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