Evaluation of Aquaporins 1 and 5 Expression in Rat Parotid Glands After Volumetric Modulated Arc Radiotherapy and Use of Low-Level Laser Therapy at Different Times
| Autor: | Daniel Simon, Humberto Thomazi Gassen, Milene Castilhos de Oliveira, Pedro Antonio González Hernández, Juliana Tomaz Sganzerla, Sergio Augusto Quevedo Miguens-Jr, Gabriel Francisco Krueger, Ivana Grivicich |
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| Rok vydání: | 2020 |
| Předmět: |
Arc (protein)
business.industry Urology medicine.medical_treatment Aquaporin 030206 dentistry Dermatology Right parotid gland Radiation therapy 03 medical and health sciences 0302 clinical medicine Real-time polymerase chain reaction Nephrology 030220 oncology & carcinogenesis Gene expression medicine Dentistry (miscellaneous) Orthopedics and Sports Medicine Surgery Original Article Nuclear medicine business Rat Parotid Low level laser therapy |
| Zdroj: | J Lasers Med Sci |
| ISSN: | 2008-9783 |
| Popis: | Introduction: This experimental study investigated the mRNA expression of aquaporins (AQPs) 1 and 5 in the parotid glands of rats irradiated with volumetric modulated arc therapy (VMAT) and subjected to low-level laser therapy (LLLT) at different time points. Methods: The sample consisted of 30 Wistar rats (Rattus norvegicus) divided into the following groups: control, LLLT alone (LG), radiotherapy alone (RG), and experimental groups that received LLLT at 24 hours (early experimental group [EEG], n=12) and 120 hours (late experimental group [LEG], n=12) after radiotherapy. VMAT was delivered at a single dose (12 Gy) and LLLT was performed with an aluminium-gallium-arsenide diode laser (660 nm, 100 mW), spot area of 0.0028 cm2, energy of 2 J/cm2 applied to 3 spots in the region corresponding to the right parotid gland, for 10 consecutive days. The right parotid gland was resected and prepared for RNA extraction. The gene expression of AQPs was evaluated by quantitative polymerase chain reaction (qPCR) using specific TaqMan probes, with the HPRT gene as an internal control. Results: The lowest AQP1 gene expression was 0.83 (0.27) with the use of LLLT 24 hours after radiotherapy (EEG), and the highest was 1.56 (0.80) with the use of LLLT alone (LG). Likewise, the lowest AQP5 gene expression was found in the EEG (mean = 0.88; SD = 0.49) and the highest in the LG (mean = 1.29; SD = 0.33). Conclusion: The use of LLLT after radiotherapy may contribute to the maintenance and an increase of these proteins, even when used at a later time point after radiotherapy. |
| Databáze: | OpenAIRE |
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