Use of cyclic strain bioreactor for the upregulation of key tenocyte gene expression on Poly(glycerol-sebacate) (PGS) sheets
| Autor: | Selcan Guler, Ekin Çelik, Halil Murat Aydin, Pezhman Hosseinian, Pelin Deniz |
|---|---|
| Přispěvatelé: | Kırşehir Ahi Evran Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri, Tıbbi Mikrobiyoloji ABD |
| Rok vydání: | 2020 |
| Předmět: |
Glycerol
Materials science Cell Survival Polymers Bioreactor Biocompatible Materials Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials Extracellular matrix Bioreactors Tissue engineering Tendon cell Human tenocytes Gene expression medicine Humans Viability assay Poly(glycerol-sebacate) (PGS) Cells Cultured Mechanical stimulation Tissue Scaffolds Regeneration (biology) Decanoates 021001 nanoscience & nanotechnology Up-Regulation 0104 chemical sciences Tendon Cell biology Tenocytes medicine.anatomical_structure Mechanics of Materials Cell culture Transcriptome 0210 nano-technology |
| Zdroj: | Materials Science and Engineering: C. 106:110293 |
| ISSN: | 0928-4931 |
| Popis: | The inadequate donor source and the difficulty of using natural grafts in tendon repair and regeneration has led researchers to develop biodegradable and biocompatible synthetic based tissue equivalents. Poly(glycerol sebacate) (PGS) is a surface-erodible bioelastomer and has been increasingly investigated in a variety of biomedical applications. In this study, PGS elastomeric sheets were prepared by using a facile microwave method and used as elastomeric platform for the first time under mechanical stimulation to induct the tenocyte gene expression. It is revealed that elastomeric PGS sheets promote progenitor tendon cell structure by increasing proliferation and gene expression with regard to tendon extracellular matrix components. Human tenocytes were seeded onto poly(glycerol-sebacate) sheets and were cultured two days prior to transfer to dynamic culture in a bioreactor system. Cell culture studies were carried out for 12 days under 0%, 3% and 6% strain at 0.33 Hz. The PGS-cell constructs were examined by using Scanning Electron Microscopy (SEM), cell viability via live/dead staining using confocal microscopy, and GAG/DNA analysis. In addition, gene expression was examined using real-time polymerase chain reaction (RT-PCR). Tenocytes cultured upon PGS scaffolds under 6% cyclic strain exhibited tendon-like gene expression profile compared to 3% and 0% strain groups. The results of this study show that PGS is a suitable material in promoting tendon tissue formation under dynamic conditions. © 2019 Elsevier B.V. |
| Databáze: | OpenAIRE |
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