LAMP-1-chimeric DNA vaccines enhance the antibody response in Japanese flounder, Paralichthys olivaceus
| Autor: | Iang Rondón-Barragán, Ikuo Hirono, Hidehiro Kondo, Reiko Nozaki |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Aquatic Science Immunofluorescence DNA vaccination 03 medical and health sciences Fish Diseases 0302 clinical medicine Antigen Lysosomal-Associated Membrane Protein 1 Complementary DNA medicine Vaccines DNA Environmental Chemistry Animals Amino Acid Sequence Gene Edwardsiella tarda Phylogeny Expression vector medicine.diagnostic_test biology Viral Vaccines General Medicine biology.organism_classification Virology Molecular biology Olive flounder DNA Virus Infections Iridoviridae 030104 developmental biology Organ Specificity 030220 oncology & carcinogenesis Flatfishes Sequence Alignment |
| Zdroj: | Fishshellfish immunology. 67 |
| ISSN: | 1095-9947 |
| Popis: | DNA vaccination is one method to protect farmed fish from viral and bacterial diseases. Chimeric antigens encoded by DNA vaccines have been shown to increase the resistance to viral diseases. Here, we sequenced the gene encoding lysosome-associated membrane protein-1 from Japanese flounder, Paralichthys olivaceus, (JfLAMP-1) and assessed its use in a chimeric DNA vaccine fused with the major capsule protein (MCP) from red seabream iridovirus (RSIV). JfLAMP-1 cDNA has a length of 1248 bp encoding 415 aa, which contains transmembrane and cytoplasmic domains. JfLAMP-1 is constitutively expressed in several tissues and its expression in spleen was upregulated following injection of formalin-killed cells (FKC) of Edwardsiella tarda. Immunofluorescence analysis showed that JfLAMP-1 is distributed in the small and large granules in the cytoplasm and groups close to the nucleus. The DNA encoding the luminal domain of JfLAMP-1 was replaced with the gene for the RSIV MCP, and the construct was cloned in an expression vector (pCIneo). Fish vaccinated with pCLAMP-MCP had significantly higher antibody levels than fish vaccinated with pCIneo vector harboring the MCP gene (p < 0.05) at day 30 post-vaccination. |
| Databáze: | OpenAIRE |
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