An MspI polymorphism in the inhibin alpha gene and its associations with superovulation traits in Chinese Holstein cows
| Autor: | Ke-Qiong Tang, Li Han, Xiang Li, Shu-Jing Li, Wu-Cai Yang, Liguo Yang, Jun-Na Yu |
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| Rok vydání: | 2010 |
| Předmět: |
China
medicine.medical_specialty Genotype Superovulation Quantitative trait locus Biology Deoxyribonuclease HpaII Quantitative Trait Heritable Polymorphism (computer science) Internal medicine Genetics medicine Animals Inhibins Molecular Biology Genetic Association Studies Genetic association Polymorphism Genetic INHA food and beverages General Medicine Embryo Transfer Embryo Mammalian Embryo transfer Endocrinology Fertilization Cattle Female Gene polymorphism Follicle Stimulating Hormone Restriction fragment length polymorphism |
| Zdroj: | Molecular Biology Reports. 38:17-21 |
| ISSN: | 1573-4978 0301-4851 |
| Popis: | To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, the PCR–RFLP method was applied to detect an A>G transition determining an MspI polymorphism at position 192 in the exon I of the bovine inhibin alpha (INHA) gene and evaluate its associations with superovulatory response in 118 Chinese Holstein cows treated for superovulation. Association analysis showed that cows with the GG genotype resulted in a significant increase in the number of ova (TNO) than AG and AA genotypes in the first (P = 0.023), second (P = 0.004) and third (P = 0.002) superovulation treatments and produced more transferable embryos (NTE) than that of AG and AA genotypes in the third (P = 0.045) superovulation treatment. Moreover, individuals with GG genotype produced more transferable embryos than AA (P < 0.05) genotype in the second superovulation treatment and all cows without superovulation response were mutations with genotypes of AA and AG. These results indicate that INHA gene can be used as a predictor for superovulation in Chinese Holstein cows, and imply that cows with AA genotype should be excluded for superovulation practices. |
| Databáze: | OpenAIRE |
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