RNA sequencing of Brassica napus reveals cellular redox control of Sclerotinia infection
| Autor: | Ian J. Girard, Chaobo Tong, de Kievit T, Huang J, Mark F. Belmonte, Mao X, Liu S, Michael G. Becker, Fernando Wgd |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine oilseed rape Physiology Mutant RNA-Seq Plant Science Biology 01 natural sciences 03 medical and health sciences Ascomycota RNA seq Arabidopsis Botany Gene Regulatory Networks Pathogen Gene Plant Diseases Genetics disease Plant Stems Sequence Analysis RNA plant-pathogen interactions Sclerotinia sclerotiorum Brassica napus RNA food and beverages Ethylenes biology.organism_classification Research Papers Plant Leaves 030104 developmental biology Crop Molecular Genetics redox Host-Pathogen Interactions transcription factor network Sclerotinia Oxidation-Reduction 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 |
| Popis: | Protection against Sclerotinia sclerotiorum in Brassica napus is mediated via dynamic transcription factor networks and cellular redox homeostasis directly at the site of infection. Brassica napus is one of the world’s most valuable oilseeds and is under constant pressure by the necrotrophic fungal pathogen, Sclerotinia sclerotiorum, the causal agent of white stem rot. Despite our growing understanding of host pathogen interactions at the molecular level, we have yet to fully understand the biological processes and underlying gene regulatory networks responsible for determining disease outcomes. Using global RNA sequencing, we profiled gene activity at the first point of infection on the leaf surface 24 hours after pathogen exposure in susceptible (B. napus cv. Westar) and tolerant (B. napus cv. Zhongyou 821) plants. We identified a family of ethylene response factors that may contribute to host tolerance to S. sclerotiorum by activating genes associated with fungal recognition, subcellular organization, and redox homeostasis. Physiological investigation of redox homeostasis was further studied by quantifying cellular levels of the glutathione and ascorbate redox pathway and the cycling enzymes associated with host tolerance to S. sclerotiorum. Functional characterization of an Arabidopsis redox mutant challenged with the fungus provides compelling evidence into the role of the ascorbate-glutathione redox hub in the maintenance and enhancement of plant tolerance against fungal pathogens. |
| Databáze: | OpenAIRE |
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