Separation of glucose and pentose sugars by selective enzyme hydrolysis of AFEX-treated corn fiber
Autor: | Robert J. Hanchar, Mark D. Stowers, Farzaneh Teymouri, Chandra Nielson, Darold McCalla |
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Rok vydání: | 2008 |
Předmět: |
Arabinose
Bioengineering Cellulase Xylose Chemical Fractionation Applied Microbiology and Biotechnology Biochemistry Zea mays Hydrolysate Hydrolysis chemistry.chemical_compound Ammonia Enzymatic hydrolysis Organic chemistry Hemicellulose Molecular Biology Chromatography biology food and beverages General Medicine Xylan Glucose chemistry biology.protein Biotechnology |
Zdroj: | Applied biochemistry and biotechnology. (1-12) |
ISSN: | 1559-0291 |
Popis: | A process was developed to fractionate corn fiber into glucose- and pentose-rich fractions. Corn fiber was ammonia fiber explosion treated at 90 degrees C, using 1 g anhydrous ammonia pergram of drybiomass, 60% moisture, and 30-min residence time. Twenty four hour hydrolysis of ammonia fiber explosion-treated corn fiber with cellulase converted 83% of available glucanto-glucose. In this hydrolysis the hemicellulose was partially broken down with 81% of the xylan and 68% of the arabinan being contained in the hydrolysate after filtration to remove lignin and other insoluble material. Addition of ethanol was used to precipitate and recover the solubilized hemicellulose from the hydrolysate, followed by hydrolysis with 2% (v/v) sulfuric acid to convert the recovered xylan and arabinan to monomeric sugars. Using this method, 57% of xylose and 54% of arabinose available in corn fiber were recovered in a pentose-rich stream. The carbohydrate composition of the pentose-enriched stream was 5% glucose, 57% xylose, 27% arabinose, and 11% galactose. The carbohydrate composition of the glucose-enriched stream was 87% glucose, 5% xylose, 6% arabinose, and 1% galactose, and contained 83% of glucose available from the corn fiber. |
Databáze: | OpenAIRE |
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