An autocrine role for endothelin-1 in the regulation of proximal tubule NHE3
| Autor: | Christoph Licht, Robert J. Alpern, Masashi Yanagisawa, Kamel Laghmani, Patricia A. Preisig |
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| Rok vydání: | 2004 |
| Předmět: |
Male
chronic metabolic acidosis medicine.medical_specialty Kidney Cortex Sodium-Hydrogen Exchangers Kidney Glomerulus Cell heart In Vitro Techniques Kidney Tubules Proximal Rats Sprague-Dawley Internal medicine medicine Animals RNA Messenger Autocrine signalling Na+/H+ antiporter Acidosis Messenger RNA Endothelin-1 Sodium-Hydrogen Exchanger 3 urogenital system Chemistry Myocardium Chronic metabolic acidosis Cardiac muscle Metabolic acidosis medicine.disease autocrine regulation Endothelin 1 Rats Autocrine Communication aorta Endocrinology medicine.anatomical_structure Nephrology Chronic Disease medicine.symptom |
| Zdroj: | Kidney International. 65:1320-1326 |
| ISSN: | 0085-2538 |
| Popis: | An autocrine role for endothelin-1 in the regulation of proximal tubule NHE3. Background Chronic metabolic acidosis leads to an increase in NHE3 activity that is mediated by endothelin-1 (ET-1) expression and activation of the proximal tubule endothelin B receptor. Chronic metabolic acidosis increases preproET-1 mRNA abundance in kidney cortex, but the cell responsible has not been identified. Methods PreproET-1 mRNA abundance was quantified by competitive reverse transcription-polymerase chain reaction (RT-PCR) on tissue harvested from control rats or rats in which chronic metabolic acidosis was induced by addition of NH 4 Cl to the drinking water. Results Chronic metabolic acidosis leads to an increase in preproET-1 mRNA expression in kidney cortex, proximal tubules, and glomeruli. The increase in preproET-1 expression correlates with the decrease in blood [HCO 3 − ]. ET-1 expression is also increased by acidosis in abdominal aorta, but not in cardiac muscle. Conclusion In the renal proximal tubule, chronic metabolic acidosis induces an increase in preproET-1 expression, providing a mechanism for autocrine regulation of proximal tubule NHE3 activity. This response is not unique to the proximal tubule cell, but is also not ubiquitous. |
| Databáze: | OpenAIRE |
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