Chemical characteristics of an Ilex Kuding tea polysaccharide and its protective effects against high fructose-induced liver injury and vascular endothelial dysfunction in mice
| Autor: | Xichuan Zhai, Daoyuan Ren, Yiyang Luo, Xingbin Yang, Yuanyuan Hu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Endothelium Fructose Ilex medicine.disease_cause Mice 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Polysaccharides Enos Malondialdehyde Internal medicine medicine Animals Humans Oil Red O Vascular Diseases Endothelial dysfunction Liver injury Glutathione Peroxidase biology Plant Extracts Superoxide Dismutase business.industry 04 agricultural and veterinary sciences General Medicine medicine.disease biology.organism_classification 040401 food science Fatty Liver Oxidative Stress 030104 developmental biology Endocrinology medicine.anatomical_structure Liver chemistry Immunology Endothelium Vascular Steatosis business Oxidative stress Food Science |
| Zdroj: | Food & Function. 8:2536-2547 |
| ISSN: | 2042-650X 2042-6496 |
| Popis: | The present study was designed to investigate the protective effects of Ilex Kuding tea polysaccharides (IKTP) on high fructose (HF)-induced liver injury and vascular endothelial dysfunction in mice. IKTP were identified as acidic heteropolysaccharides by FT-IR and HPLC. Healthy male Kunming mice fed 20% fructose in drinking water for 8 consecutive weeks significantly displayed dyslipidemia, hepatic steatosis, oxidative stress and vascular endothelial dysfunction. However, continuous administration of IKTP at 200, 400 and 800 mg per kg bw in HF-fed mice could prevent the damage caused by HF-diets, especially at dosages of 400 and 800 mg per kg bw (p < 0.01). IKTP significantly reduced the HF-induced elevation of the serum TC, TG, LDL-C, TXA2 and ET-1 levels, as well as AST and ALT activities, while markedly increased the HF-induced decline of HDL-C, PGI2 and eNOS levels in the serum compared to HF-fed mice. Meanwhile, the hepatic MDA level was lowered while SOD and GSH-Px activities were increased in IKTP-treated mice, compared to HF-fed mice. Histopathology of the liver and cardiovascular aortic by H&E or oil red O staining confirmed the liver steatosis and the vascular injury induced by HF-diets and the protective effects of IKTP. These findings suggest that HF causes oxidative damage, and IKTP alleviates liver injury and vascular endothelial dysfunction. |
| Databáze: | OpenAIRE |
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