Delivery of polysaccharides from Ophiopogon japonicus (OJPs) using OJPs/chitosan/whey protein co-assembled nanoparticles to treat defective intestinal epithelial tight junction barrier

Autor: Fwu Long Mi, Jing Chi Lin, Yi Cheng Ho, Chi Lin, Tai Chih Kuo
Rok vydání: 2020
Předmět:
Whey protein
Antioxidant
medicine.medical_treatment
Ophiopogon japonicus
Administration
Oral
Gene Expression
02 engineering and technology
Pharmacology
Biochemistry
Chitosan
Mice
chemistry.chemical_compound
Drug Delivery Systems
Structural Biology
Intestinal Mucosa
Cytotoxicity
Drug Carriers
0303 health sciences
Tight junction
biology
General Medicine
021001 nanoscience & nanotechnology
Intestines
Paracellular transport
medicine.symptom
0210 nano-technology
Biological Availability
Inflammation
Permeability
Cell Line
Tight Junctions
03 medical and health sciences
Polysaccharides
Cell Line
Tumor
medicine
Animals
Humans
Molecular Biology
030304 developmental biology
Macrophages
Ophiopogon
Epithelial Cells
biology.organism_classification
RAW 264.7 Cells
Whey Proteins
chemistry
Nanoparticles
Caco-2 Cells
Zdroj: International Journal of Biological Macromolecules. 160:558-570
ISSN: 0141-8130
DOI: 10.1016/j.ijbiomac.2020.05.151
Popis: The polysaccharides from Ophiopogon japonicus (OJPs) were known to have protective effects against diabetes, and cardiovascular and chronic inflammatory diseases. However, OJPs were poorly absorbed after oral administration, resulting in limited efficacy because of the low bioavailability. In this study, OJPs extracted and fractionated from Ophiopogon japonicus were used to prepare OJPs/chitosan (CS)/whey protein (WP) co-assembled nanoparticles. The OJPs/CS/WP nanoparticles showed high biocompatibility and inhibited the cytotoxicity of RAW264.7 cells induced by nickel. With the assistance of CS and WP, the anti-inflammatory and antioxidant activities of OJPs were enhanced because the nanoparticles improved OJPs uptake by RAW264.7 macrophage cells as evidenced by efficient scavenging of DPPH and ABTS free radicals and effective inhibition of NO production and the gene expressions of iNOS, COX2, TNF-α, CCL2, and CXCL2 inflammatory signals. Determining the transepithelial electrical resistance and paracellular permeability of Caco-2 monolayer/macrophage co-cultured system suggested that the OJPs-loaded nanoparticles effectively protected the intestinal epithelial barrier integrity against the damage caused by LPS-stimulated macrophage inflammation and attenuated the defects of intestinal epithelial TJ barrier and permeability. These findings suggest that the OJPs/CS/WP nanoparticles may be potential carriers for oral delivery of OJPs to treat intestinal barrier defects, such as inflammatory bowel disease (IBD).
Databáze: OpenAIRE
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