The EF-hand Ca2+ binding protein MICU choreographs mitochondrial Ca2+ dynamics in Arabidopsis

Autor: Wagner, S, Behera, S, De Bortoli, S, Logan, DC, Fuchs, P, Carraretto, L, Teardo, E, Cendron, L, Nietzel, T, Füßl, M, Doccula, FG, Navazio, L, Fricker, MD, Van Aken, O, Finkemeier, I, Meyer, AJ, Szabò, I, Costa, A, Schwarzländer, M
Přispěvatelé: Department of Mathematical Sciences [Matieland, Stellenbosch Uni.] (DMS), Stellenbosch University, Institut de Recherche en Horticulture et Semences (IRHS), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Centre Européen de Réalité Virtuelle (CERV), École Nationale d'Ingénieurs de Brest (ENIB), Rheinische Friedrich-Wilhelms-Universität Bonn, Max Planck Gesellschaft, University of Padova through Progetto di Ateneo, Ministero dell'Istruzione, dell'Universita e della Ricerca through the PRIN project [2010CSJX4F], FIRB programme [RBFR10S1LJ_001], Deutsche Forschungsgemeinschaft through the Emmy-Noether programme [SCHW1719/1-1], Université d'Angers (UA)-AGROCAMPUS OUEST-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Europe PubMed Central
The Plant cell
The Plant cell, American Society of Plant Biologists (ASPB), 2015, 27 (11), pp.3190-3212. ⟨10.1105/tpc.15.00509⟩
Plant and Cell Physiology
Plant Cell
Plant Cell, American Society of Plant Biologists, 2015, 27 (11), pp.3190-3212. ⟨10.1105/tpc.15.00509⟩
The Plant cell 27 (2015): 3190–3212. doi:10.1105/tpc.15.00509
info:cnr-pdr/source/autori:Wagner S.; Behera S.; De Bortoli S.; Logan D.C.; Fuchs P.; Carraretto L.; Teardo E.; Cendron L.; Nietzel T.; Fussl M.; Doccula F.G.; Navazio L.; Fricker M.D.; Van Aken O.; Finkemeier I.; Meyer A.J.; Szabo I.; Costa A.; Schwarzlander M./titolo:The EF-hand Ca2+ binding protein MICU choreographs mitochondrial Ca2+ dynamics in arabidopsis/doi:10.1105%2Ftpc.15.00509/rivista:The Plant cell/anno:2015/pagina_da:3190/pagina_a:3212/intervallo_pagine:3190–3212/volume:27
ISSN: 1040-4651
1532-298X
DOI: 10.1105/tpc.15.00509
Popis: Plant organelle function must constantly adjust to environmental conditions, which requires dynamic coordination. Ca2+ signaling may play a central role in this process. Free Ca2+ dynamics are tightly regulated and differ markedly between the cytosol, plastid stroma, and mitochondrial matrix. The mechanistic basis of compartment-specific Ca2+ dynamics is poorly understood. Here, we studied the function of At-MICU, an EF-hand protein of Arabidopsis thaliana with homology to constituents of the mitochondrial Ca2+ uniporter machinery in mammals. MICU binds Ca2+ and localizes to the mitochondria in Arabidopsis. In vivo imaging of roots expressing a genetically encoded Ca2+ sensor in the mitochondrial matrix revealed that lack of MICU increased resting concentrations of free Ca2+ in the matrix. Furthermore, Ca2+ elevations triggered by auxin and extracellular ATP occurred more rapidly and reached higher maximal concentrations in the mitochondria of micu mutants, whereas cytosolic Ca2+ signatures remained unchanged. These findings support the idea that a conserved uniporter system, with composition and regulation distinct from the mammalian machinery, mediates mitochondrial Ca2+ uptake in plants under in vivo conditions. They further suggest that MICU acts as a throttle that controls Ca2+ uptake by moderating influx, thereby shaping Ca2+ signatures in the matrix and preserving mitochondrial homeostasis. Our results open the door to genetic dissection of mitochondrial Ca2+ signaling in plants.
Databáze: OpenAIRE
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