Analysis of human neutrophil phenotypes as biomarker to monitor exercise-induced immune changes
Autor: | Roy Spijkerman, Nienke Vrisekoop, Jeroen J. Jansen, Lillian Hesselink, Falco Hietbrink, Carlo Bertinetto, Luke P. H. Leenen, Leo Koenderman, Maria T. E. Hopman, Coen C. W. G. Bongers |
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Rok vydání: | 2021 |
Předmět: |
Male
0301 basic medicine Neutrophils Visual analogue scale Receptor expression Immunology Vascular damage Radboud Institute for Health Sciences [Radboudumc 16] innate immune system Walking Biology Article Fluorescence Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Immune system Antigens CD medicine Humans Immunology and Allergy Exercise Aged Innate immune system repetitive exercise prolonged walking Overtraining Metabolic Disorders Radboud Institute for Health Sciences [Radboudumc 6] Translational and Clinical Immunology neutrophil Cell Biology Middle Aged medicine.disease Immunity Innate Blood Cell Count Phenotype 030104 developmental biology 030220 oncology & carcinogenesis Cohort Exercise intensity Biomarker (medicine) Female Biomarkers |
Zdroj: | Journal of Leukocyte Biology, 109, 4, pp. 833-842 Journal of Leukocyte Biology, 109, 833-842 Journal of Leukocyte Biology |
ISSN: | 0741-5400 |
Popis: | The amplitude of the innate immune response reflects the degree of physiological stress imposed by exercise load. An optimal balance of exercise intensity and duration is essential for a balanced immune system and reduces the risk of dysfunction of the immune system. Therefore, it is hypothesized that neutrophils, as key players in the innate immune system, can be used as biomarker in detecting overtraining. The aim was to monitor the state of the innate immune system by phenotyping neutrophils during consecutive bouts of prolonged exercise. Study subjects were recruited from a cohort of walkers participating in a walking event on 3 consecutive days. Participants with immune deficiencies were excluded. Questionnaires to determine the physiological status of the participants were completed. Analysis of neutrophil receptor expression was done by a point‐of‐care fully automated flow cytometer. A total of 45 participants were recruited, of whom 39 participants were included for data analysis. Study participants had a median age of 64 (58‐70) years. The absolute numbers CD16dim/CD62Lbright and CD16bright/CD62Ldim neutrophils were increased after the first 2 days of exercise followed by an adaptation/normalization after the third day. Participants with activated neutrophils (high CD11b expression) had an impaired physical feeling indicated by the participant on a lower visual analog scale compared to participants who did not have activated neutrophils (P = 0.017, P = 0.022). Consecutive days of prolonged exercise results in an initial systemic innate immune response, followed by normalization/adaptation. Increased neutrophil activation was associated with impaired physical feeling measured by a validated VAS score indicated by the participant. Fully automated point‐of‐care flow cytometry analysis of neutrophil phenotypes in a field laboratory might be a useful tool to monitor relevant differences in the systemic innate immune response in response to exercise. Graphical Abstract Fully automated flow cytometry analysis of neutrophils obtained in a field laboratory after repetitive prolonged exercise. |
Databáze: | OpenAIRE |
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