Modified Western blot assay for confirmation and differentiation of human T cell lymphotropic virus types I and II
| Autor: | Paul Santiago, K. P. Samuel, George M. Shaw, C. V. Hanson, Steven K. H. Foung, Gregory R. Reyes, Lily Chan, John J. Sninsky, J. J. Lipka, Blattner Wa |
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| Rok vydání: | 1991 |
| Předmět: |
Radioimmunoprecipitation Assay
viruses Blotting Western Virus law.invention Western blot immune system diseases law hemic and lymphatic diseases medicine Immunology and Allergy Humans False Positive Reactions Antiserum Human T-lymphotropic virus 1 biology medicine.diagnostic_test Immune Sera Human T-lymphotropic virus 2 virus diseases Group-specific antigen biology.organism_classification Virology Molecular biology HTLV-I Infections HTLV-I Antibodies HTLV-II Antibodies Infectious Diseases HTLV-II Infections biology.protein Recombinant DNA Antibody |
| Zdroj: | The Journal of infectious diseases. 164(2) |
| ISSN: | 0022-1899 |
| Popis: | Disease association studies of human T cell lymphotropic virus (HTLV) types I and II are hindered by the need for multiple assays to confirm and differentiate between the viruses. A modified Western blot assay has been developed using HTLV-I viral lysate and unique (MTA-4) and shared (p21E) HTLV recombinant proteins. By defining confirmation of infection as the presence of antibodies to p24 gag protein and to p21E, all 56 HTLV-I and 49 HTLV-II antisera were confirmed by this modified Western blot alone. Differentiation was determined by reactivity to MTA-4. All HTLV-I antisera reacted with MTA-4 and all HTLV-II antisera did not react with MTA-4. These findings indicate the utility of selected HTLV-I recombinant proteins in a single assay format to confirm and differentiate infections with HTLV-I and HTLV-II. |
| Databáze: | OpenAIRE |
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