Preparation and characterization of lipid vesicles containing uricase
| Autor: | Huarong Xiong, Jun Liu, Linghuan Zhang, Qunyou Tan, Chunjing Zhao, N Wang, Jiye Zhang, Li Chen, H. Yang |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Urate Oxidase Surface Properties Drug Compounding Drug Storage Pharmaceutical Science Rats Sprague-Dawley chemistry.chemical_compound In vivo Enzyme Stability Zeta potential medicine Animals Hyperuricemia Particle Size chemistry.chemical_classification Drug Carriers Chromatography Proteolytic enzymes General Medicine medicine.disease In vitro Rats Enzyme chemistry Biochemistry Phosphatidylcholines Uric acid Particle size |
| Zdroj: | Drug Delivery. 17:28-37 |
| ISSN: | 1521-0464 1071-7544 |
| DOI: | 10.3109/10717540903508953 |
| Popis: | The study described the development of lipid vesicles as colloidal carriers for uricase, an enzyme with low activity at physicological conditions and low stability in vitro and in vivo. The lipid vesicles containing uricase (UOXLVs) were prepared and the process parameters were optimized with the indexes of entrapment efficiency, polydispersion, particle size, and zeta potential. The storage stability of uricase in lipid vesicles was significantly increased compared to that of free uricase at 4°C. The stability to proteolytic digestion was also increased obviously by entrapping the uricase in the lipid vesicles. In vitro and in vivo pharmacodynamic studies on the hyperuricemia rat model explicitly suggested that the uricase entrapped by UOXLVs possessed high uricolytic activity and distinctively decreased the uric acid level. |
| Databáze: | OpenAIRE |
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