Optimization of antigen-specific CD8+ T cell activation conditions for infectious diseases including COVID-19
| Autor: | Jieping Deng, Oscar Junhong Luo, Congling Qiu, Lijuan Gao, Jun Su, Guobing Chen, Pengcheng Wang, Chanchan Xiao, Jiezhou Ye |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Artificial antigen
Science (General) General Immunology and Microbiology Coronavirus disease 2019 (COVID-19) Chemistry General Neuroscience Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Cell Immunology Cell Biology Molecular biology Microbiology General Biochemistry Genetics and Molecular Biology Q1-390 medicine.anatomical_structure Tetramer Antigen specific Cell isolation medicine Cytotoxic T cell CD8 |
| Zdroj: | STAR Protocols, Vol 2, Iss 3, Pp 100789-(2021) |
| ISSN: | 2666-1667 |
| Popis: | Summary: Here, we describe the use of the artificial antigen-presenting cell (aAPC) system for the verification of T-cell epitopes. We purify and activate CD8+ T cells from blood samples from HLA-A2 that are negative for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). CD8+ T cells are combined with peptide-loaded T2-A2 cells, which are then stained with a SARS-CoV-2-specific MHC-1 tetramer to identify specific HLA-A2-restricted T-cell epitopes. The use of aAPC and healthy donors means that only BSL2 lab conditions are needed.For details of the use and implementation of this protocol, please refer to Deng et al. (2021). |
| Databáze: | OpenAIRE |
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