Precision-Cut Bovine Udder Slices (PCBUS) as an in-vitro-model of an early phase of infection of bovine mastitis
| Autor: | Monique Petry, Jessica Meißner, Viviane Filor, Manfred Kietzmann |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
In-vitro-model 600 Technik Medizin angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche 030106 microbiology Cytological Techniques Inflammation In Vitro Techniques Models Biological In vitro model 03 medical and health sciences Antibiotic resistance Mammary Glands Animal Medicine Animals Secretion Udder Mastitis Bovine Bovine mastitis lcsh:Veterinary medicine General Veterinary business.industry General Medicine Antimicrobial medicine.disease Precision-cut bovine udder slices Mastitis Cytokine profile 030104 developmental biology medicine.anatomical_structure Immunology Cytokines lcsh:SF600-1100 Cattle Female medicine.symptom business Early phase Research Article |
| Zdroj: | BMC Veterinary Research, Vol 17, Iss 1, Pp 1-9 (2021) BMC Veterinary Research |
| Popis: | Background The aim of this study was to establish precision-cut bovine udder slices (PCBUS) as an in-vitro-model to investigate pathophysiological processes in the early phase of mastitis in order to have the possibility to investigate new therapeutic approaches for the treatment of such udder inflammation in later studies. Furthermore, this model should contribute to substitute in-vivo-experiments. Bovine mastitis is one of the most common and costly infectious diseases in the dairy industry, which is largely associated with the use of antimicrobial agents. Given this problem of antimicrobial resistance, it is essential to step up research into bacterial infectious diseases. Thus, the transfer of the in-vitro-model of precision-cut tissue slices to the bovine udder enables broad research into new therapeutic approaches in this area and can also be used to address issues in basic research or the characterisation of complex pathophysiological processes. Results A stimulation with LPS, PGN or the combination of both substances (LPS:PGN) demonstrates the ability of the PCBUS to react with a significant secretion of IL-1ß, TNF-α and PGE2. Conclusion The slices represent an instrument for investigating pharmacological interactions with udder tissue, which can be useful for studies on pharmacological questions and the understanding of complex pathophysiological processes of infection and inflammation. |
| Databáze: | OpenAIRE |
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