Quantitation of serum 25(OH)D2 and 25(OH)D3 concentrations by liquid chromatography tandem mass spectrometry in patients with diabetes mellitus
| Autor: | Hei-Hwa Lee, Yung-Hung Lee, Shing-Cheng Tseng, Kun-Der Lin, Li-Ping Tseng, Yi-Ching Lin, Jong-Feng Lee, Bai-Hsiun Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male endocrine system diseases 030309 nutrition & dietetics Electrospray ionization chemistry.chemical_element lcsh:TX341-641 Type 2 diabetes Calcium 01 natural sciences Young Adult 03 medical and health sciences Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Diabetes mellitus medicine Vitamin D and neurology Humans In patient Vitamin D Chromatography High Pressure Liquid Pharmacology 0303 health sciences Type 1 diabetes Chromatography 010401 analytical chemistry lcsh:RM1-950 nutritional and metabolic diseases Middle Aged medicine.disease 0104 chemical sciences Diabetes Mellitus Type 1 lcsh:Therapeutics. Pharmacology Diabetes Mellitus Type 2 chemistry Case-Control Studies Female lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Journal of Food and Drug Analysis, Vol 27, Iss 2, Pp 510-517 (2019) |
| ISSN: | 1021-9498 |
| Popis: | Vitamin D has been considered to regulate calcium and phosphorus homeostasis and to preserve skeletal integrity. Serum 25-hydroxyvitamin D (25(OH)D) is the best indicator of vitamin D levels. The association of serum 25(OH)D deficiency with increased risk of type 1 diabetes (T1DM) and type 2 diabetes (T2DM) is controversial. We investigated serum 25(OH)D2 and 25(OH)D3 levels in diabetes patients by using liquid chromatography tandem mass spectrometry (LC-MS/MS). Serum 25(OH)D2 and 25(OH)D3 levels were measured with liquid chromatography tandem mass spectrometry in electrospray ionization positive mode. Chromatograms were separated using an ACE5 C18 column on a gradient of methanol. The total 25(OH)D levels were calculated as the sum of 25(OH)D3 and 25(OH)D2 levels. A total of 56 patients with T1DM and 41 patients with T2DM were enrolled in this study. There were 42 and 28 non-diabetic, age-matched volunteers who participated as the T1DM controls and the T2DM controls, respectively. The total 25(OH)D levels were lowest in the 21–40 age group. The levels of both 25(OH)D3 and the total 25(OH)D were significantly higher in the T1DM and T2DM groups than in the controls (p |
| Databáze: | OpenAIRE |
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