Identification of rep-associated factors in herpes simplex virus type 1-induced adeno-associated virus type 2 replication compartments
| Autor: | Lauriane Kuhn, Armel Nicolas, Anna Salvetti, Coline Biollay, Loredana Arata, Alberto L. Epstein, Nelly Jolinon, Sandra K. Weller, Anna Greco, Nathalie Alazard-Dany, Myriam Ferro |
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| Přispěvatelé: | Centre de génétique et de physiologie moléculaire et cellulaire (CGPhiMC), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Laboratoire de physiologie cellulaire végétale (LPCV), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie à Grande Échelle (BGE - UMR S1038), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
viruses
MESH: DNA Replication MESH: Herpes Simplex Herpesvirus 1 Human MESH: Dependovirus medicine.disease_cause Virus Replication Replication Protein A Chlorocebus aethiops Protein Interaction Mapping MESH: Animals Replication Protein C Adeno-Associated Virus Type 2 0303 health sciences biology 030302 biochemistry & molecular biology Dependovirus Genome Replication and Regulation of Viral Gene Expression DNA-Binding Proteins MESH: Herpesvirus 1 Human Helper virus DNA mismatch repair Human Protein Binding DNA Replication Immunology MESH: Vero Cells MESH: Parvoviridae Infections Microbiology Virus Cercopithecus aethiops Parvoviridae Infections 03 medical and health sciences Viral Proteins Virology medicine Animals Humans MESH: Protein Binding MESH: Replication Protein C [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology MESH: Replication Protein A Vero Cells 030304 developmental biology MESH: Humans Herpesvirus 1 MESH: Protein Interaction Mapping MESH: Virus Replication DNA replication Herpes Simplex MESH: Cercopithecus aethiops MESH: Viral Proteins Proliferating cell nuclear antigen Herpes simplex virus Viral replication Insect Science MESH: HeLa Cells biology.protein MESH: DNA-Binding Proteins HeLa Cells |
| Zdroj: | Journal of Virology Journal of Virology, American Society for Microbiology, 2010, 84 (17), pp.8871-87. ⟨10.1128/JVI.00725-10⟩ Journal of Virology, 2010, 84 (17), pp.8871-87. ⟨10.1128/JVI.00725-10⟩ |
| ISSN: | 0022-538X 1098-5514 |
| DOI: | 10.1128/JVI.00725-10⟩ |
| Popis: | Adeno-associated virus (AAV) is a human parvovirus that replicates only in cells coinfected with a helper virus, such as adenovirus or herpes simplex virus type 1 (HSV-1). We previously showed that nine HSV-1 factors are able to support AAV rep gene expression and genome replication. To elucidate the strategy of AAV replication in the presence of HSV-1, we undertook a proteomic analysis of cellular and HSV-1 factors associated with Rep proteins and thus potentially recruited within AAV replication compartments (AAV RCs). This study resulted in the identification of approximately 60 cellular proteins, among which factors involved in DNA and RNA metabolism represented the largest functional categories. Validation analyses indicated that the cellular DNA replication enzymes RPA, RFC, and PCNA were recruited within HSV-1-induced AAV RCs. Polymerase δ was not identified but subsequently was shown to colocalize with Rep within AAV RCs even in the presence of the HSV-1 polymerase complex. In addition, we found that AAV replication is associated with the recruitment of components of the Mre11/Rad50/Nbs1 complex, Ku70 and -86, and the mismatch repair proteins MSH2, -3, and -6. Finally, several HSV-1 factors were also found to be associated with Rep, including UL12. We demonstrated for the first time that this protein plays a role during AAV replication by enhancing the resolution of AAV replicative forms and AAV particle production. Altogether, these analyses provide the basis to understand how AAV adapts its replication strategy to the nuclear environment induced by the helper virus. |
| Databáze: | OpenAIRE |
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