Enzyme-Linked Phage Receptor Binding Protein Assays (ELPRA) Enable Identification of Bacillus anthracis Colonies

Autor:	Diana Neif, Peter Braun, Nadja Rupprich, Gregor Grass
Rok vydání:	2021
Předmět:	reporter fusions Bacillus receptor binding protein Microbiology law.invention Bacteriophage ELPRA 03 medical and health sciences bacteriophage law Virology Centrifugation Bacillus anthracis Polymerase chain reaction 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology 030306 microbiology enzyme-linked phage protein assay anthrax biology.organism_classification QR1-502 Infectious Diseases Enzyme chemistry Biochemistry Recombinant DNA Bacteria
Zdroj:	Viruses Volume 13 Issue 8 Viruses, Vol 13, Iss 1462, p 1462 (2021)
ISSN:	1999-4915
DOI:	10.3390/v13081462
Popis:	Bacteriophage receptor binding proteins (RBPs) are employed by viruses to recognize specific surface structures on bacterial host cells. Recombinant RBPs have been utilized for detection of several pathogens, typically as fusions with reporter enzymes or fluorescent proteins. Identification of Bacillus anthracis, the etiological agent of anthrax, can be difficult because of the bacterium’s close relationship with other species of the Bacillus cereussensu lato group. Here, we facilitated the identification of B. anthracis using two implementations of enzyme-linked phage receptor binding protein assays (ELPRA). We developed a single-tube centrifugation assay simplifying the rapid analysis of suspect colonies. A second assay enables identification of suspect colonies from mixed overgrown solid (agar) media derived from the complex matrix soil. Thus, these tests identified vegetative cells of B. anthracis with little processing time and may support or confirm pathogen detection by molecular methods such as polymerase chain reaction.
Databáze:	OpenAIRE
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