Enterotoxigenic Bacteroides fragilis infection exacerbates tumorigenesis in AOM/DSS mouse model
| Autor: | Samnoh Hwang, Ki Jong Rhee, Yong-Bin Eom, Chan Oh Park, Minjeong Jo, So-Yeon Lee, Sun Yeong Gwon, Soonjae Hwang, Chang Gun Lee, Baktiar Karim, Hye Chin Yi |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Colorectal cancer
Carcinogenesis Colon Bacterial Toxins Azoxymethane Inflammation colorectal cancer Biology medicine.disease_cause digestive system ETBF Microbiology Bacteroides fragilis 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Polyps medicine otorhinolaryngologic diseases Animals Humans Colitis Dextran Sulfate Cancer Metalloendopeptidases General Medicine medicine.disease biology.organism_classification Bacteroides Infections digestive system diseases Disease Models Animal chemistry inflammation dextran sulfate sodium 030211 gastroenterology & hepatology medicine.symptom Colorectal Neoplasms Bacteria Research Paper |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| Popis: | The azoxymethane (AOM)/dextran sulfate sodium (DSS) murine model is commonly used to study colitis-associated cancer. The human commensal bacterium, enterotoxigenic Bacteroides fragilis (ETBF) secretes the Bacteroides fragilis toxin (BFT) which is necessary and sufficient to cause colitis. We report that BALB/c mice infected with WT-ETBF and administered three cycles of AOM/DSS developed numerous, large-sized polyps predominantly in the colorectal region. In addition, AOM/DSS-treated BALB/c mice orally inoculated with wild-type nontoxigenic Bacteroides fragilis (WT-NTBF) overexpressing bft (rETBF) developed numerous polyps whereas mice infected with WT-NTBF overexpressing a biologically inactive bft (rNTBF) did not promote polyp formation. Unexpectedly, the combination of AOM+ETBF did not induce polyp formation whereas ETBF+DSS did induce polyp development in a subset of BALB/c mice. In conclusion, WT-ETBF promoted polyp development in AOM/DSS murine model with increased colitis in BALB/c mice. The model described herein provides an experimental platform for understanding ETBF-induced colonic tumorigenesis and studying colorectal cancer in wild-type mice. |
| Databáze: | OpenAIRE |
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