Isophosphoramide mustard analogues as prodrugs for anticancer gene-directed enzyme-prodrug therapy (GDEPT)
| Autor: | Misiura K, Szymanowicz D, Kuśnierczyk H, Joanna Wietrzyk, Opolski A |
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| Rok vydání: | 2002 |
| Předmět: |
Leukemia
Nucleotides Carcinoma Esterases Antineoplastic Agents Esters General Biochemistry Genetics and Molecular Biology Mice Cytochrome P-450 Enzyme System Drug Stability Tumor Cells Cultured Animals Humans Mouth Neoplasms Phosphoramide Mustards Prodrugs Ifosfamide Chromatography High Pressure Liquid |
| Zdroj: | Scopus-Elsevier Europe PubMed Central |
| ISSN: | 1734-154X 0001-527X |
| DOI: | 10.18388/abp.2002_3833 |
| Popis: | Two types of prodrugs, benzyl analogues of isophosphoramide mustard (iPAM), activated by cytochrome P450, and acylthioethyl analogues, activated by esterases, were designed. In contrast to iPAM that hydrolyse rapidly, the examined compounds are stable in phosphate-buffered saline and Tris buffer. Benzyl analogues of iPAM are poor substrates for cytochrome P450, are not cytotoxic and posses no antitumour activity. Acylthioethyl analogues of iPAM are good substrates for pig liver esterase, are cytotoxic and exert antitumour activity against L1210 leukaemia in mice. The observed correlation for iPAM analogues between their susceptibility to hydrolysis and cytotoxicity and antitumour activity suggests possible application of these compounds as the prodrugs in gene-directed enzyme-prodrug therapy. |
| Databáze: | OpenAIRE |
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