Inflammatory response of cultured rat synoviocytes challenged with synovial fluid from osteoarthritis patients correlates with their radiographic grading: a pilot study
| Autor: | Soumya J. Koppikar, Dattatray Shinde, Abhay Harsulkar, Alpana Moghe, Dhanashri R Ingale, Shantanu Deshpande, Priya Kulkarni, Prabhakar K. Ranjekar, Narendrakumar Wagh |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Lipopolysaccharide Interleukin-1beta Inflammation Pilot Projects Osteoarthritis Nitric Oxide Severity of Illness Index Nitric oxide Glycosaminoglycan Andrology chemistry.chemical_compound Synovial Fluid medicine Potency Synovial fluid Animals Humans Rats Wistar Cells Cultured Aged Glycosaminoglycans business.industry Cell Biology General Medicine Middle Aged medicine.disease Rats Radiography chemistry Cell culture Immunology Female medicine.symptom business Developmental Biology |
| Zdroj: | In vitro cellulardevelopmental biology. Animal. 51(8) |
| ISSN: | 1543-706X |
| Popis: | The inflammatory nature of synovial fluid (SF) of varying grade osteoarthritis (OA) patients was estimated by measuring pro-inflammatory factors and through a unique cell-challenge experiment. SF samples were collected from six OA and one non-OA patient; spanning Kellgren-Lawrence (KL) grades were analyzed for interlukin-1-beta (IL-1β), nitric oxide (NO) and its derivatives, and glycosaminoglycan (GAG). Levels of IL-1β, NO, and GAG in SF did not correlate with KL grades of the patients studied. In the cell-challenge experiment, cultured rat synoviocyte fibroblasts (RSFs) were challenged by the patient's SFs with and without pre-treatment of IL-1β and lipopolysaccharide (LPS). NO released by the cells was taken as an indicator of inflammation. SFs from KL grades 2 and 3 induced maximum inflammation in cultured RSFs (grade 2 64.61 ± 4.8 and 89.51 ± 5.6 μM/ml after 48 and 72 h, grade 3 58.27 ± 2.7 and 64.22 ± 2.8 μM/ml after 48 and 72 h, respectively). Similar trend was observed in RSF pretreated with either recombinant IL-1β or LPS suggesting that SF from patients KL grades 2 and 3 accumulates more pro-inflammatory factors. IL-1β-pre-treated RSFs challenged by SF for 72 h showed 234.41 ± 17.6 μM/ml increase (patient 3, grade 3), whereas higher NO after LPS pre-treatment was recorded (118.92 ± 6.2 μM/ml; patient 3, grade 3). Interestingly, SFs from grade 1 and non-OA patient could reduce released NO to 27.10 ± 2.2 μM/ml showing potency to alleviate inflammation. These interesting findings, however, need to be confirmed on a wider number of patients, which may offer significant therapeutic application in treatment of OA. |
| Databáze: | OpenAIRE |
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