Bushen Huoxue Huayu Recipe up-regulates expression of insulin receptor substrate-1 and phosphorylation of tyrosine in adipose tissue in androgen sterilized rats
| Autor: | Zhen-zhi Wang, Xi-lian Li, Chao-qin Yu |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Adipose tissue Rats Sprague-Dawley Random Allocation Internal medicine medicine Animals Testosterone Phosphorylation Tyrosine biology Insulin Area under the curve Rats IRS1 Insulin receptor Endocrinology Adipose Tissue Complementary and alternative medicine Insulin Receptor Substrate Proteins biology.protein Female Infertility Female Drugs Chinese Herbal Phytotherapy |
| Zdroj: | Journal of Chinese Integrative Medicine. 6:621-625 |
| ISSN: | 1672-1977 |
| DOI: | 10.3736/jcim20080615 |
| Popis: | OBJECTIVE To investigate the expression of insulin receptor substrate-1 (IRS-1) and phosphorylation of tyrosine in androgen sterilized rats (ASRs) and the effects of Bushen Huoxue Huayu Recipe (BHHR), a compound Chinese herbal medicine for tonifying kidney and activating blood, on the ASRs, and to analyze the possible mechanism. METHODS ASR models were established by means of testosterone injected subcutaneously at the age of 9 days. The model rats were randomly divided into BHHR group (n=15) and untreated group (n=15); the rats in normal group (n=15) were injected with oil. The rats in the BHHR group were treated with BHHR for 30 days, and the rats in model group and normal group were treated with distilled water for 30 days. Body weight and area under the curve (AUC) of insulin were measured after treatment. Expression of IRS-1 and phosphorylation of tyrosine in adipose tissue in ASRs were analysed by immunohistochemical method. RESULTS The body weight and insulin AUC in the untreated group were increased and more than those in the BHHR group and the normal control group (P |
| Databáze: | OpenAIRE |
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