Production and characterization of β‐glucosidase from Aspergillus niger fermentation: Application for organic fraction of municipal solid waste hydrolysis and methane enhancement
| Autor: | Sami Sayadi, Najoua Mlaik, Manel Hamza, Sonia Khoufi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Solid Waste 01 natural sciences Methane chemistry.chemical_compound Hydrolysis Biogas 010608 biotechnology Enzymatic hydrolysis Food science Biological Oxygen Demand Analysis biology beta-Glucosidase fungi 010401 analytical chemistry Chemical oxygen demand Aspergillus niger biology.organism_classification 0104 chemical sciences Anaerobic digestion chemistry Biofuels Fermentation Biotechnology |
| Zdroj: | Biotechnology Progress. 36 |
| ISSN: | 1520-6033 8756-7938 |
| Popis: | The anaerobic digestion of the organic fraction of municipal solid waste (OFMSW) is currently an attractive treatment process with energy production in the form of biogas. Hydrolysis is the rate-limiting step for the anaerobic digestion of solid wastes. Thus, in the present study fungal enzymatic pretreatment of OFMSW was applied to enhance biogas production. Two enzyme cocktails rich on β-glucosidase were produced from submerged fermentation of Aspergillus niger on basal medium using OFMSW as carbon source and urea (Urea cocktail) and Ulva rigida as nitrogen source (Ulva cocktail). Ulva cocktail displayed an important effect on OFMSW solubilization. Therefore, an increase of reducing sugar concentration about 60% was obtained which was in correlation with chemical oxygen demand (COD) increase. The performance of enzymatic pretreatment on anaerobic digestion of OFMSW was studied by conducting biochemical methane potential tests. Results showed that the enzymatic pretreatment improved methane yield of OFMSW even at high solid concentration. High methane yield about 500 ml/g total volatile solid was obtained, which corresponds up to 68% enhancement over the control. |
| Databáze: | OpenAIRE |
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