Probing spermatogenesis in Drosophila with P-element enhancer detectors

Autor: Stephen DiNardo, S. Viswanathan, Pierre Gönczy
Rok vydání: 1992
Předmět:
Genetic Markers
Male
Cell type
Somatic cell
Lac Operon/genetics
Spermatozoa/*cytology/physiology/ultrastructure
Drosophila melanogaster/*embryology
Fluorescent Antibody Technique
Gene Expression
Embryoid body
Biology
Research Support
Electron
Mesoderm
Testis
medicine
Cell Differentiation/physiology
Animals
Microscopy
Phase-Contrast
Spermatogenesis/*physiology
Gene Expression/physiology
Non-U.S. Gov't
Spermatogenesis
Molecular Biology
Germ plasm
Genetics
Microscopy
Phase-Contrast
Cell Differentiation
Mesoderm/*cytology
Immunohistochemistry
Spermatozoa
Cell biology
Microscopy
Electron
Drosophila melanogaster
Enhancer Elements
Genetic
medicine.anatomical_structure
Stem cell division
Lac Operon
Enhancer Elements (Genetics)/genetics
Testis/cytology/ultrastructure
Germ line development
Stem cell
Germ cell
Developmental Biology
Zdroj: Scopus-Elsevier
Europe PubMed Central
ISSN: 1477-9129
0950-1991
DOI: 10.1242/dev.114.1.89
Popis: Formation of motile sperm in Drosophila melanogaster requires the coordination of processes such as stem cell division, mitotic and meiotic control and structural reorganization of a cell. Proper execution of spermatogenesis entails the differentiation of cells derived from two distinct embryonic lineages, the germ line and the somatic mesoderm. Through an analysis of homozygous viable and fertile enhancer detector lines, we have identified molecular markers for the different cell types present in testes. Some lines label germ cells or somatic cyst cells in a stage-specific manner during their differentiation program. These expression patterns reveal transient identities for the cyst cells that had not been previously recognized by morphological criteria. A marker line labels early stages of male but not female germ cell differentiation and proves useful in the analysis of germ line sex-determination. Other lines label the hub of somatic cells around which germ line stem cells are anchored. By analyzing the fate of the somatic hub in an agametic background, we show that the germ line plays some role in directing its size and its position in the testis. We also describe how marker lines enable us to identify presumptive cells in the embryonic gonadal mesoderm before they give rise to morphologically distinct cell types. Finally, this collection of marker lines will allow the characterization of genes expressed either in the germ line or in the soma during spermatogenesis.
Databáze: OpenAIRE
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