Epithelial cell motility is triggered by activation of the EGF receptor through phosphatidic acid signaling
| Autor: | Hewa B. Achebe, Ethan R. Block, Jes K. Klarlund, Julie K. Spix, Abigail R. Mazie |
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| Rok vydání: | 2006 |
| Předmět: |
Phosphatidic Acids
In Vitro Techniques Biology Models Biological chemistry.chemical_compound Transactivation Dogs Cell Movement Epidermal growth factor Phosphatidylcholine Lysophosphatidic acid Phospholipase D Animals Humans Cells Cultured Protein Kinase C Diacylglycerol kinase Wound Healing Dose-Response Relationship Drug Epidermal Growth Factor PLD2 Epithelium Corneal Cell Biology Phosphatidic acid Cell biology Enzyme Activation ErbB Receptors chemistry Rabbits Cell Adhesion Molecules Signal Transduction |
| Zdroj: | Journal of Cell Science. 119:1645-1654 |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.02858 |
| Popis: | Phospholipase D catalyzes the hydrolysis of phosphatidylcholine to generate phosphatidic acid, and there is currently much interest in elucidating messenger functions for this molecule. We report here that wounding sheets of corneal epithelial and Madin Darby canine kidney cells induces strong activation of phospholipase D, and we provide evidence that activation is amplified through a positive feed-back loop. Short-chain analogues of phosphatidic acid induce motility robustly in corneal and other epithelial cell types. The effects of these analogues were not the result of their conversion to the corresponding diacylglycerol or lysophosphatidic acid, implying that phosphatidic acid acts directly on one or more cellular targets. Strikingly, phosphatidic acid signaling was found to stimulate the epidermal growth factor receptor (EGFR) through a transactivation process. Healing of wounds in sheets of corneal epithelial cells is absolutely dependent on epidermal growth factor receptor signaling, and the present data suggest that its activation is a result of wound-induced phospholipase D activation. |
| Databáze: | OpenAIRE |
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