Isolated rat hepatocyte metabolism is affected by chronic renal failure
| Autor: | Xavier Leverve, Noël Cano, Robert Novaretti, F. Catelloni, Jean Pierre Reynier, Jeanne di Costanzo-Dufetel, Eric Fontaine |
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| Přispěvatelé: | Service d'Hépatogastroentérologie et Nutrition, Clinique Résidence du Parc, Bioénergétique fondamentale et appliquée, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Chimie Biologique, Hôpital de la Timone [CHU - APHM] (TIMONE), Hamant, Sarah |
| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty MESH: Rats 030309 nutrition & dietetics MESH: Adenine Nucleotides Cell Separation Ketone Bodies Biology MESH: Cell Separation Nephropathy 03 medical and health sciences Oxygen Consumption Adenine nucleotide Internal medicine Ketogenesis medicine [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Animals Urea MESH: Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology MESH: Oxygen Consumption Rats Wistar MESH: Urea 030304 developmental biology 2. Zero hunger 0303 health sciences Adenine Nucleotides MESH: Ketone Bodies MESH: Energy Metabolism Gluconeogenesis MESH: Gluconeogenesis Metabolism MESH: Rats Wistar medicine.disease MESH: Male Rats Cytosol medicine.anatomical_structure Endocrinology Liver Glycogenesis MESH: Kidney Failure Chronic Nephrology Hepatocyte Kidney Failure Chronic Energy Metabolism MESH: Liver |
| Zdroj: | Kidney International Kidney International, Nature Publishing Group, 1995, 47 (6), pp.1522-7 Scopus-Elsevier |
| ISSN: | 0085-2538 1523-1755 |
| DOI: | 10.1038/ki.1995.215 |
| Popis: | Isolated rat hepatocyte metabolism is affected by chronic renal failure. Metabolic changes due to chronic renal failure (CRF) were studied in isolated liver cells. In 14 CRF and 14 sham-operated rats, liver cells were isolated by the Berry and Friend method and incubated with various substrates in order to study gluconeogenesis, ureagenesis, ketogenesis, oxygen consumption as well as cytosolic and mitochondrial adenine nucleotide content. CRF rat hepatocytes exhibited a 25% to 45% decrease in gluconeogenesis and ureagenesis (P < 0.05) from all the tested substrates (lactate plus pyruvate, fructose, glycerol, dihydroxyacetone, alanine and glutamine for gluconeogenesis and alanine, glutamine, ammonia and ammonia plus ornithine for ureagenesis), while endogenous rates were unaffected. CRF did not alter ketone body production (acetoacetate and β-hydroxybutyrate) from oleate or octanoate. In the presence of either oleate, lactate plus pyruvate or ammonia, oxygen uptake as well as cytosolic and mitochondrial total adenine nucleotides were unaffected by CRF, while the mitochondrial ATP/ADP ratio decreased (P < 0.001). Thus, this study of hepatocyte intermediary metabolism during CRF showed an alteration of only gluconeogenesis and ureagenesis pathways. Moreover, the association of normal oxygen uptake together with decreased mitochondrial ATP/ADP ratio suggest a possible increase in hepatocyte ATP demand during uremia. |
| Databáze: | OpenAIRE |
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