A synthetic biology approach allows inducible retrotransposition in whole plants
| Autor: | Andreas Bachmair, Gudrun Böhmdorfer, Andrea Tramontano, Kerstin Luxa |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Genetics
Arabidopsis thaliana Systems biology Methodology Article fungi food and beverages Retrotransposon Bioengineering Biology Plants Genome Long terminal repeat Insertional mutagenesis Transposition (music) Synthetic biology Tto1 Transposon mutagenesis Molecular Biology Inducible promoter Biotechnology |
| Zdroj: | Systems and Synthetic Biology |
| Popis: | Retrotransposons are mobile genetic elements that transpose by reverse transcription of element RNA, followed by insertion of the cDNA into new positions of the host genome. Although they are major constituents of eukaryotic genomes, many facets of their biology remain to be understood. Transposition is generally rare, suggesting that it is subject to tight regulation. However, only the first regulatory step (transcriptional induction) is currently amenable to investigation in higher eukaryotes. To investigate the complete life cycle of a long terminal repeat (LTR) retrotransposon in plants, we established a synthetic biology program on tobacco retrotransposon Tto1, and achieved transposition in whole plants triggered by an inducible promoter. The engineered element, iTto (inducible Tto1), is a novel tool for analysis of retrotransposition in plants. In addition, it allows to explore the potential of an inducible retrotransposon for insertional mutagenesis. Electronic supplementary material The online version of this article (doi:10.1007/s11693-010-9053-4) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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