Mutagenesis in ORF AV2 affects viral replication in Mungbean yellow mosaic India virus
| Autor: | A. Rouhibakhsh, Q. M. I. Haq, V. G. Malathi |
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| Rok vydání: | 2011 |
| Předmět: |
Mutant
Molecular Sequence Data Mutation Missense Mutagenesis (molecular biology technique) Virus Replication General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Open Reading Frames Amino Acid Sequence Gene Genetics Phaseolus biology Base Sequence Pigmentation Begomovirus Wild type General Medicine biology.organism_classification Virology Plant Leaves chemistry Viral replication Mutagenesis Site-Directed DNA supercoil General Agricultural and Biological Sciences DNA |
| Zdroj: | Journal of biosciences. 36(2) |
| ISSN: | 0973-7138 |
| Popis: | Mungbean yellow mosaic India virus (MYMIV) is a whitefly-transmitted begomovirus with a bipartite genome. We investigate the functions of the MYMIV-AV2 protein, the open reading frame present upstream of the coat protein gene in DNA A component. The ability of MYMIV-AV2 mutants to replicate, spread and cause symptoms in legume hosts, blackgram, cowpea and French bean was analysed. Plants agroinoculated with mutants K73R, C86S and the double mutant C84S,C86S showed increase in severity of symptoms compared with the wild type. However, mutants W2S and H14Q,G15E caused marked attenuation of symptoms. While the double mutants C84S,C86S caused a 50-fold increase in double-stranded supercoiled and single-stranded DNA accumulation, the mutations W2S and H14Q,G15E showed a decrease in double-stranded supercoiled and single-stranded viral DNA accumulation. Because AV2 mutants affect the ratio between open circular and supercoiled DNA forms, we hypothesize that these mutations may modulate the functions of the replication initiation protein. |
| Databáze: | OpenAIRE |
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