Lower expression of the equine maternally imprinted gene IGF2R is related to the slow proliferation of hinny embryonic fibroblast in vitro

Autor: Xisheng Wang, Nairag Asgenbaatar, Yingchao Shen, Minna Yi, Bilig Zhao, Hong Ren, Toli Davshilt, Tseweendolmaa Ulaangerel, Min Wang, Als Burenbaatar, Shuyue Tian, Bei Li, Manglai Dugarjav, Gerelchimeg Bou
Rok vydání: 2022
Předmět:
Zdroj: Molecular Biology Reports. 50:185-192
ISSN: 1573-4978
0301-4851
DOI: 10.1007/s11033-022-07937-6
Popis: Proliferation of embryonic fibroblasts under the same cell culture conditions, hinny embryonic fibroblasts (HiEFs) was slower than horse embryonic fibroblast (HEFs), donkey embryonic fibroblasts (DEFs) and mule embryonic fibroblasts (MuEFs). The imprinted genes IGF2 and IGF2R are important for cell proliferation. Therefore, we investigated whether the slower proliferation of HiEFs is related to an aberrant gene expression of IGF2 or its receptors or genes influencing the expression of the IGF2 system.Real-time polymerase chain reaction, immunofluorescence and cell starving experiment in HEFs, DEFs, MuEFs and HiEFs revealed that the slower proliferation of HiEF in vitro was related to its lower expression of IGF2R (P 0.001). Moreover, quantification of allele-specific expression and bisulfate assay confirmed that in both MuEFs and HiEFs, IGF2R had normal maternal imprinting, implying that the imprint aberrant was not involved in the lower IGF2R expression in HiEFs.The reduction of IGF2R expression in HiEFs is associated with its slower proliferation in vitro.
Databáze: OpenAIRE
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