Ameliorative effect of betulinic acid on oxidative damage and apoptosis in the splenocytes of dexamethasone treated mice
| Autor: | Jianping Wu, Ruocen Zhu, Jing Wu, Jine Yi, Zhuliang Tan |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Antioxidant
Necrosis Cell Survival medicine.medical_treatment Immunology Apoptosis Mice Inbred Strains Mitochondrion Pharmacology medicine.disease_cause Dexamethasone Lipid peroxidation chemistry.chemical_compound Mice Betulinic acid medicine Immunology and Allergy Animals Betulinic Acid chemistry.chemical_classification Reactive oxygen species Caspase 3 Anti-Inflammatory Agents Non-Steroidal Triterpenes Mitochondria Oxidative Stress chemistry Biochemistry Proto-Oncogene Proteins c-bcl-2 Lipid Peroxidation medicine.symptom Pentacyclic Triterpenes Reactive Oxygen Species Oxidative stress Spleen |
| Zdroj: | International immunopharmacology. 27(1) |
| ISSN: | 1878-1705 |
| Popis: | Betulinic acid (BA) is a bioactive pentacyclic triterpene that exhibits a variety of biological activities including antioxidative and immunomodulative properties. The objective of this study was to investigate the potential splenocytes protective effect and underlying mechanism of BA using dexamethasone (Dex)-induced mice as a model system. Pretreatment with BA (0.25, 0.5, and 1.0 mg/kg) dose-dependently ameliorated Dex-induced oxidative damage and apoptosis after 14 days of feeding. In addition to reactive oxygen species scavenging activity in Dex-induced splenocytes, BA administration up-regulated antioxidant enzymes, decreased lipid peroxidation, restored mitochondrial function, decreased the expression of pro-apoptotic protein Bax, prevented the decline of anti-apoptotic protein Bcl-2, inhibited caspase-9 and caspase-3 activation, and improved cell survival. These findings reveal that BA was able to mitigate Dex-induced oxidative stress and might play an important role in repairs of oxidative damage in immunological system. |
| Databáze: | OpenAIRE |
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