Changes in cecal microbial metabolism of rats induced by individual and a mixture of drinking water disinfection by-products
| Autor: | S. E. George, K. C. Bailey, L. R. Brooks, D.C. Wolf, Michelle J. Hooth, Gail M. Nelson |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Cancer Research Heterozygote Microbial metabolism Bromodichloromethane Nitroreductase chemistry.chemical_compound Tuberous Sclerosis Complex 2 Protein Animals NADH NADPH Oxidoreductases Rats Long-Evans Food science Furans Cecum Glucuronidase Chloroform Bacteria Chemistry Bromates Tumor Suppressor Proteins beta-Glucosidase Body Weight Nitroreductases Bromate beta-Galactosidase Rats Repressor Proteins Drug Combinations Oncology Distilled water Biochemistry Xenobiotic Potassium bromate Water Pollutants Chemical Mutagens Trihalomethanes |
| Zdroj: | Cancer letters. 204(1) |
| ISSN: | 0304-3835 |
| Popis: | Disinfection of drinking water has been one of the greatest public health successes. Numerous halogenated disinfection by-products (DBPs) occur and chronic ingestion has been associated with an increased risk for colorectal cancer in human populations. Because the intestinal microbiota can bioactivate xenobiotics, studies have been performed to examine the effects of individual DBPs on intestinal microbial metabolism. No studies have been conducted on a defined mixture of DBPs to determine if there is an enhancement of response to a mixture. Ten-week-old male Long-Evans rats were treated in their drinking water for 17 weeks with 0.4 g/l potassium bromate, 1.8 g/l chloroform, 0.7 g/l bromodichloromethane (BDCM), 0.07 g/l 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX), or a mixture of the four chemicals or distilled water. Cecal nitroreductase (NR), azoreductase (AR), dechlorinase (DC), beta-glucuronidase (GLR), beta-galactosidase (GAL), and beta-glucosidase (GLU) were assayed. No change in GLU or GLR activity was detected after treatment. BDCM treatment reduced DC and GAL activities and elevated NR and AR activity. GAL, AR, and NR activities were significantly different after treatment with bromate, chloroform, BDCM, and MX, but not the mixture. DC activity after chloroform-, MX-, or BDCM-treatment was significantly below control levels. The present study shows that changes in intestinal microbial metabolism do occur after treatment with individual and a mixture of DBPs but the changes were not additive in the mixture group. |
| Databáze: | OpenAIRE |
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