A 3-dimensional microfluidic platform for modeling human extravillous trophoblast invasion and toxicological screening
Autor: | Jeremy Gingrich, Yong Pu, Almudena Veiga-Lopez |
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Rok vydání: | 2021 |
Předmět: |
Placenta
Microfluidics Biomedical Engineering Bioengineering Biochemistry Article Flow cytometry Extracellular matrix 03 medical and health sciences 0302 clinical medicine Cell Movement Pregnancy medicine Humans 030304 developmental biology Cell invasion 0303 health sciences medicine.diagnostic_test Chemistry Trophoblast General Chemistry Silicone polymer Trophoblasts Cell biology medicine.anatomical_structure 030220 oncology & carcinogenesis Extravillous trophoblast embryonic structures Female |
Zdroj: | Lab Chip |
ISSN: | 1473-0189 1473-0197 |
Popis: | Placental trophoblast cells invasion into the maternal uterus is an essential and complex event in the formation of the maternal-fetal interface. Commonly used two-dimensional (2D) cell invasion tools do not accurately represent the in vivo cell invasion microenvironment. Three-dimensional (3D) silicone polymer polydimethylsiloxane (PDMS) microfluidic platforms are an emerging technology in developing organ-on-a-chip models. Here, we present a placenta-on-a-chip platform that enables the evaluation of trophoblast invasion with intraluminal flow within an engineered PDMS 3D microfluidic chip. This platform reproduces key elements of the placental microenvironment, including endothelial and trophoblast cells, layered with an extracellular matrix, and incorporates dynamic medium flow while allowing for real-time monitoring, imaging, evaluation of trophoblast cell invasion, and heterocellular cell-to-cell interactions. Coupled with fluorescent cell tagging and flow cytometry, this platform also allows collection of the invasive cells. This will help our understanding of pathways that regulate trophoblast cell invasion and may prove important for toxicological screening of exposures that interfere with invasiveness in a complex organ such as the placenta. |
Databáze: | OpenAIRE |
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